1yf4
From Proteopedia
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|PDB= 1yf4 |SIZE=350|CAPTION= <scene name='initialview01'>1yf4</scene>, resolution 1.98Å | |PDB= 1yf4 |SIZE=350|CAPTION= <scene name='initialview01'>1yf4</scene>, resolution 1.98Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yf4 OCA], [http://www.ebi.ac.uk/pdbsum/1yf4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yf4 RCSB]</span> | ||
}} | }} | ||
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[[Category: Ibrahim, B Syed.]] | [[Category: Ibrahim, B Syed.]] | ||
[[Category: Pattabhi, V.]] | [[Category: Pattabhi, V.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: NH2]] | ||
[[Category: peptide binding]] | [[Category: peptide binding]] | ||
[[Category: trypsin]] | [[Category: trypsin]] | ||
[[Category: vasopressin]] | [[Category: vasopressin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:05:20 2008'' |
Revision as of 22:05, 30 March 2008
| |||||||
| , resolution 1.98Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Trypsin, with EC number 3.4.21.4 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of trypsin-vasopressin complex
Overview
The large variety of serine protease inhibitors, available from various sources such as tissues, microorganisms, plants, etc., play an important role in regulating the proteolytic enzymes. The analysis of protease-inhibitor complexes helps in understanding the mechanism of action, as well as in designing inhibitors. Vasopressin, an anti-diuretic nonapeptide hormone, is found to be an effective inhibitor of trypsin, with a K(i) value of 5 nM. The crystal structure of the trypsin-vasopressin complex revealed that vasopressin fulfils all the important interactions for an inhibitor, without any break in the scissile peptide bond. The cyclic nature due to a disulfide bridge between Cys1 and Cys6 of vasopressin provides structural rigidity to the peptide hormone. The trypsin-binding site is located at the C terminus, while the neurophysin-binding site is at the N terminus of vasopressin. This study will assist in designing new peptide inhibitors. This study suggests that vasopressin inhibition of trypsin may have unexplored biological implications.
About this Structure
1YF4 is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
Trypsin inhibition by a peptide hormone: crystal structure of trypsin-vasopressin complex., Syed Ibrahim B, Pattabhi V, J Mol Biol. 2005 May 20;348(5):1191-8. Epub 2005 Apr 1. PMID:15854654
Page seeded by OCA on Mon Mar 31 01:05:20 2008
