5mih
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the lectin LecA from Pseudomonas aeruginosa in complex with a phenyl-epoxy-galactopyranoside== | |
| + | <StructureSection load='5mih' size='340' side='right' caption='[[5mih]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5mih]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MIH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MIH FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7NU:Phenyl+6,7-dideoxy-6,7-epoxy-beta-D-galacto-heptopyranoside(6D)'>7NU</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ETE:2-{2-[2-2-(METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>ETE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mih OCA], [http://pdbe.org/5mih PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mih RCSB], [http://www.ebi.ac.uk/pdbsum/5mih PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mih ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/PA1L_PSEAE PA1L_PSEAE]] D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Biofilm formation by pathogenic bacteria is a hallmark of chronic infections. In many cases, lectins play key roles in establishing biofilms. The pathogen Pseudomonas aeruginosa often exhibiting various drug resistances employs its lectins LecA and LecB as virulence factors and biofilm building blocks. Therefore, inhibition of the function of these proteins is thought to have potential in developing 'pathoblockers' preventing biofilm formation and virulence. Here, we describe for the first time a covalent lectin inhibitor specific to a carbohydrate binding site. In addition we report its application in the LecA-specific in vitro imaging of biofilms formed by P. aeruginosa. | ||
| - | + | Covalent lectin inhibition and application in bacterial biofilm imaging.,Wagner S, Hauck D, Hoffmann M, Sommer R, Joachim I, Muller R, Imberty A, Varrot A, Titz A Angew Chem Int Ed Engl. 2017 Sep 28. doi: 10.1002/anie.201709368. PMID:28960731<ref>PMID:28960731</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5mih" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Hauk, D]] | [[Category: Hauk, D]] | ||
| - | [[Category: Joachim, I]] | ||
[[Category: Hofmann, M]] | [[Category: Hofmann, M]] | ||
| - | [[Category: Titz, A]] | ||
[[Category: Imberty, A]] | [[Category: Imberty, A]] | ||
| + | [[Category: Joachim, I]] | ||
| + | [[Category: Muller, R]] | ||
| + | [[Category: Sommer, R]] | ||
| + | [[Category: Titz, A]] | ||
| + | [[Category: Varrot, A]] | ||
| + | [[Category: Wagner, S]] | ||
| + | [[Category: Covalent lectin inhibition]] | ||
| + | [[Category: Galactose binding protein]] | ||
| + | [[Category: Lectin]] | ||
| + | [[Category: Sugar binding protein]] | ||
Revision as of 06:48, 11 October 2017
Crystal structure of the lectin LecA from Pseudomonas aeruginosa in complex with a phenyl-epoxy-galactopyranoside
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