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1jza

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Revision as of 07:33, 11 October 2017

Crystal Structure of Variant 2 Scorpion Toxin from Centruroides sculpturatus Ewing

Structural highlights

1jza is a 2 chain structure with sequence from Bark scorpion. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1jzb
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SCX2_CENSC] Beta toxins bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing (By similarity). Induces immediate paralysis in crickets after injection, with a total paralysis occurring within 15-30 minutes and lasting for 1-2 hours. Is also lethal to vertebrate (chicks) when injected in very high dosages (more that 100 mg/kg).^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Centruroides sculpturatus Ewing variant 2 toxin (CsE-v2) is a neurotoxin isolated from the venom of a scorpion native to the Arizona desert. The structure of CsE-v2 was solved in two different crystal forms using a combination of molecular replacement and multiple isomorphous replacement techniques. Crystals of CsE-v2 display a temperature-dependent, reversible-phase transition near room temperature. At lower temperature the space group changes from P3(2)21 to P3(1)21 with an approximate doubling of the C-axis. The small-cell structure, which has one molecule per asymmetric unit, has an R factor of 0.229 at 2.8 A resolution. The large-cell structure has two molecules per asymmetric unit and was refined at 2.2 A resolution to an R factor of 0.255. CsE-v2 is a rigid, compact structure with four intrachain disulfide bonds. The structure is similar to other long-chain beta neurotoxins, and the largest differences occur in the last six residues. The high-resolution structure of CsE-v2 corrects an error in the reported C-terminal sequence; the terminal tripeptide sequence is Ser 64-Cys 65-Ser 66 rather than Ser 64-Ser 65-Cys 66. Comparison of CsE-v2 with long-chain alpha toxins reveals four insertions and one deletion, as well as additional residues at the N and C termini. Structural alignment of alpha and beta toxins suggests that the primary distinguishing feature between the two classes is the length of the loop between the second and third strands in a three-strand beta sheet. The shorter loop in alpha toxins exposes a critical lysine side chain, whereas the longer loop in beta toxins buries the corresponding basic residue (either arginine or lysine).

Structure of variant 2 scorpion toxin from Centruroides sculpturatus Ewing.,Cook WJ, Zell A, Watt DD, Ealick SE Protein Sci. 2002 Mar;11(3):479-86. PMID:11847271^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Babin DR, Watt DD, Goos SM, Mlejnek RV. Amino acid sequences of neurotoxic protein variants from the venom of Centruroides sculpturatus Ewing. Arch Biochem Biophys. 1974 Oct;164(2):694-706. PMID:4460885
↑ Cook WJ, Zell A, Watt DD, Ealick SE. Structure of variant 2 scorpion toxin from Centruroides sculpturatus Ewing. Protein Sci. 2002 Mar;11(3):479-86. PMID:11847271

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jza"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Variant 2 Scorpion Toxin from Centruroides sculpturatus Ewing==
 <StructureSection load='1jza' size='340' side='right' caption='[[1jza]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1jza]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Centruroides_sculpturatus Centruroides sculpturatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JZA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1jza]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bark_scorpion Bark scorpion]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JZA FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1jzb|1jzb]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jza OCA], [http://pdbe.org/1jza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jza RCSB], [http://www.ebi.ac.uk/pdbsum/1jza PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jza OCA], [http://pdbe.org/1jza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jza RCSB], [http://www.ebi.ac.uk/pdbsum/1jza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jza ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jza ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Centruroides sculpturatus]]
+[[Category: Bark scorpion]]
 [[Category: Cook, W J]]
 [[Category: Ealick, S E]]

1jza

From Proteopedia

Revision as of 07:33, 11 October 2017

Crystal Structure of Variant 2 Scorpion Toxin from Centruroides sculpturatus Ewing

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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