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1u5w

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Revision as of 08:35, 12 October 2017

Crystal structure of hypothetical protein yjjX from Escherichia coli

Structural highlights

1u5w is a 8 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	yjjX ("Bacillus coli" Migula 1895)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

[NCPP_ECOLI] Phosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP to their respective diphosphate derivatives. Probably excludes non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions. ITP is the best substrate, followed by XTP, GDP or dITP. Also implicated in the resistance against the thiamine metabolism inhibitors bacimethrin and CF3-HMP.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Inosine triphosphate (ITP) and xanthosine triphosphate (XTP) are formed upon deamination of ATP and GTP as a result of exposure to chemical mutagens and oxidative damage. Nucleic acid synthesis requires safeguard mechanisms to minimize undesired lethal incorporation of ITP and XTP. Here, we present the crystal structure of YjjX, a protein of hitherto unknown function. The three-dimensional fold of YjjX is similar to those of Mj0226 from Methanococcus janschii, which possesses nucleotidase activity, and of Maf from Bacillus subtilis, which can bind nucleotides. Biochemical analyses of YjjX revealed it to exhibit specific phosphatase activity for inosine and xanthosine triphosphates and have a possible interaction with elongation factor Tu. The enzymatic activity of YjjX as an inosine/xanthosine triphosphatase provides evidence for a plausible protection mechanism by clearing the noncanonical nucleotides from the cell during oxidative stress in E. coli.

Identification of an ITPase/XTPase in Escherichia coli by structural and biochemical analysis.,Zheng J, Singh VK, Jia Z Structure. 2005 Oct;13(10):1511-20. PMID:16216582^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zheng J, Singh VK, Jia Z. Identification of an ITPase/XTPase in Escherichia coli by structural and biochemical analysis. Structure. 2005 Oct;13(10):1511-20. PMID:16216582 doi:10.1016/j.str.2005.07.007
↑ Zheng J, Singh VK, Jia Z. Identification of an ITPase/XTPase in Escherichia coli by structural and biochemical analysis. Structure. 2005 Oct;13(10):1511-20. PMID:16216582 doi:10.1016/j.str.2005.07.007

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1u5w"

@@ Line 1: / Line 1: @@
 ==Crystal structure of hypothetical protein yjjX from Escherichia coli==
 <StructureSection load='1u5w' size='340' side='right' caption='[[1u5w]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">yjjX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u5w OCA], [http://pdbe.org/1u5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u5w RCSB], [http://www.ebi.ac.uk/pdbsum/1u5w PDBsum], [http://www.topsan.org/Proteins/BSGI/1u5w TOPSAN]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u5w OCA], [http://pdbe.org/1u5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u5w RCSB], [http://www.ebi.ac.uk/pdbsum/1u5w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u5w ProSAT], [http://www.topsan.org/Proteins/BSGI/1u5w TOPSAN]</span></td></tr>
 </table>
 == Function ==

1u5w

From Proteopedia

Revision as of 08:35, 12 October 2017

Crystal structure of hypothetical protein yjjX from Escherichia coli

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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