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1r95

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Revision as of 10:18, 12 October 2017

Crystal Structure of IscA (native)

Structural highlights

1r95 is a 2 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1r94
Gene:	YFHF, B2528, C3053, Z3795, ECS3394, SF2575, S2747 ("Bacillus coli" Migula 1895)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ISCA_ECOLI] Is able to transfer iron-sulfur clusters to apo-ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that IscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transient iron-sulfur cluster in IscU in the presence of IscS, L-cysteine and the thioredoxin reductase system TrxA/TrxB.[HAMAP-Rule:MF_01429]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

IscA belongs to an ancient family of proteins responsible for iron-sulfur cluster assembly in essential metabolic pathways preserved throughout evolution. We report here the 2.3 A resolution crystal structure of Escherichia coli IscA, a novel fold in which mixed beta-sheets form a compact alpha-beta sandwich domain. In contrast to the highly mobile secondary structural elements within the bacterial Fe-S scaffold protein IscU, a protein which is thought to have a similar function, the great majority of the amino acids that are conserved in IscA homologues are located in elements that constitute a well-ordered fold. However, the 10-residue C-terminal tail segment that contains two invariant cysteines critical for the Fe-S-binding function of a cyanobacterial (Synechocystis PCC) IscA homologue is not ordered in our structure. In addition, the crystal packing reveals a helical assembly that is constructed from two possible tetrameric oligomers of IscA.

Crystal structure of the ancient, Fe-S scaffold IscA reveals a novel protein fold.,Bilder PW, Ding H, Newcomer ME Biochemistry. 2004 Jan 13;43(1):133-9. PMID:14705938^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bilder PW, Ding H, Newcomer ME. Crystal structure of the ancient, Fe-S scaffold IscA reveals a novel protein fold. Biochemistry. 2004 Jan 13;43(1):133-9. PMID:14705938 doi:10.1021/bi035440s

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1r95"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of IscA (native)==
 <StructureSection load='1r95' size='340' side='right' caption='[[1r95]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1r94|1r94]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">YFHF, B2528, C3053, Z3795, ECS3394, SF2575, S2747 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r95 OCA], [http://pdbe.org/1r95 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r95 RCSB], [http://www.ebi.ac.uk/pdbsum/1r95 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r95 OCA], [http://pdbe.org/1r95 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r95 RCSB], [http://www.ebi.ac.uk/pdbsum/1r95 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1r95 ProSAT]</span></td></tr>
 </table>
 == Function ==

1r95

From Proteopedia

Revision as of 10:18, 12 October 2017

Crystal Structure of IscA (native)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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