This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2b3b

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 13:00, 12 October 2017

Thermus thermophilus Glucose/Galactose Binding Protein With Bound Glucose

Structural highlights

2b3b is a 6 chain structure with sequence from Thet2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	2b3f
Gene:	ttc0328 (THET2)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Periplasmic binding proteins (PBPs) comprise a protein superfamily that is involved in prokaryotic solute transport and chemotaxis. These proteins have been used to engineer reagentless biosensors to detect natural or non-natural ligands. There is considerable interest in obtaining very stable members of this superfamily from thermophilic bacteria to use as robust engineerable parts in biosensor development. Analysis of the recently determined genome sequence of Thermus thermophilus revealed the presence of more than 30 putative PBPs in this thermophile. One of these is annotated as a glucose binding protein (GBP) based on its genetic linkage to genes that are homologous to an ATP-binding cassette glucose transport system, although the PBP sequence is homologous to periplasmic maltose binding proteins (MBPs). Here we present the cloning, over-expression, characterization of cognate ligands, and determination of the X-ray crystal structure of this gene product. We find that it is a very stable (apo-protein Tm value is 100(+/- 2) degrees C; complexes 106(+/- 3) degrees C and 111(+/- 1) degrees C for glucose and galactose, respectively) glucose (Kd value is 0.08(+/- 0.03) microM) and galactose (Kd value is 0.94(+/- 0.04) microM) binding protein. Determination of the X-ray crystal structure revealed that this T. thermophilus glucose binding protein (ttGBP) is structurally homologous to MBPs rather than other GBPs. The di or tri-saccharide ligands in MBPs are accommodated in long relatively shallow grooves. In the ttGBP binding site, this groove is partially filled by two loops and an alpha-helix, which create a buried binding site that allows binding of only monosaccharides. Comparison of ttGBP and MBP provides a clear example of structural adaptations by which the size of ligand binding sites can be controlled in the PBP super family.

The crystal structure of a thermophilic glucose binding protein reveals adaptations that interconvert mono and di-saccharide binding sites.,Cuneo MJ, Changela A, Warren JJ, Beese LS, Hellinga HW J Mol Biol. 2006 Sep 15;362(2):259-70. Epub 2006 Aug 10. PMID:16904687^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cuneo MJ, Changela A, Warren JJ, Beese LS, Hellinga HW. The crystal structure of a thermophilic glucose binding protein reveals adaptations that interconvert mono and di-saccharide binding sites. J Mol Biol. 2006 Sep 15;362(2):259-70. Epub 2006 Aug 10. PMID:16904687 doi:10.1016/j.jmb.2006.06.084

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2b3b"

@@ Line 1: / Line 1: @@
 ==Thermus thermophilus Glucose/Galactose Binding Protein With Bound Glucose==
 <StructureSection load='2b3b' size='340' side='right' caption='[[2b3b]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2b3f|2b3f]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ttc0328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3b OCA], [http://pdbe.org/2b3b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b3b RCSB], [http://www.ebi.ac.uk/pdbsum/2b3b PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3b OCA], [http://pdbe.org/2b3b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b3b RCSB], [http://www.ebi.ac.uk/pdbsum/2b3b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2b3b ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==

2b3b

From Proteopedia

Revision as of 13:00, 12 October 2017

Thermus thermophilus Glucose/Galactose Binding Protein With Bound Glucose

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox