5ufc

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'''Unreleased structure'''
 
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The entry 5ufc is ON HOLD until Paper Publication
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==Crystal Structure of Variable Lymphocyte Receptor (VLR) Tn4-22 with H-trisaccharide bound==
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<StructureSection load='5ufc' size='340' side='right' caption='[[5ufc]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5ufc]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UFC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UFC FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DR2:6-DEOXY-ALPHA-L-IDOPYRANOSYL-(1- 2)-ALPHA-D-GLUCOPYRANOSYL-(1- 4)-2-(ACETYLAMINO)-2-DEOXY-BETA-L-GULOPYRANOSE'>DR2</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5uei|5uei]], [[5uf1|5uf1]], [[5uf4|5uf4]], [[5ufb|5ufb]], [[5ufd|5ufd]], [[5uff|5uff]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ufc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ufc OCA], [http://pdbe.org/5ufc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ufc RCSB], [http://www.ebi.ac.uk/pdbsum/5ufc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ufc ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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High-quality reagents to study and detect glycans with high specificity for research and clinical applications are severely lacking. Here, we structurally and functionally characterize several variable lymphocyte receptor (VLR)-based antibodies from lampreys immunized with O erythrocytes that specifically recognize the blood group H-trisaccharide type II antigen. Glycan microarray analysis and biophysical data reveal that these VLRs exhibit greater specificity for H-trisaccharide compared with the plant lectin UEA-1, which is widely used in blood typing. Among these antibodies, O13 exhibits superior specificity for H-trisaccharide, the basis for which is revealed by comparative analysis of high-resolution VLR:glycan crystal structures. Using a structure-guided approach, we designed an O13 mutant with further enhanced specificity for H-trisaccharide. These insights into glycan recognition by VLRs suggest that lampreys can produce highly specific glycan antibodies, and are a valuable resource for the production of next-generation glycan reagents for biological and biomedical research and as diagnostics and therapeutics.
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Authors:
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Structural Insights into VLR Fine Specificity for Blood Group Carbohydrates.,Collins BC, Gunn RJ, McKitrick TR, Cummings RD, Cooper MD, Herrin BR, Wilson IA Structure. 2017 Sep 27. pii: S0969-2126(17)30294-0. doi:, 10.1016/j.str.2017.09.003. PMID:28988747<ref>PMID:28988747</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 5ufc" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Collins, B C]]
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[[Category: Cooper, M D]]
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[[Category: Cummings, R D]]
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[[Category: Gunn, R J]]
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[[Category: Herrin, B R]]
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[[Category: McKitrick, T R]]
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[[Category: Wilson, I A]]
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[[Category: Adaptive immunity]]
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[[Category: Glycan binding]]
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[[Category: Glycan receptor]]
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[[Category: Immune system]]
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[[Category: Jawless fish]]
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[[Category: Leucine-rich repeat]]
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[[Category: Lrr]]
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[[Category: Receptor]]
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[[Category: Sea lamprey]]
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[[Category: Variable lymphocyte receptor]]
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[[Category: Vlr]]

Revision as of 07:01, 18 October 2017

Crystal Structure of Variable Lymphocyte Receptor (VLR) Tn4-22 with H-trisaccharide bound

5ufc, resolution 1.89Å

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