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2gmp

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Revision as of 07:56, 18 October 2017

Metal-free (apo) P. angolensis seed lectin in complex with GlcNAC-beta(1-2)Man

Structural highlights

2gmp is a 2 chain structure with sequence from Bleedwood tree. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
NonStd Res:
Related:	1ukg, 2gme, 2gmm, 2gn3, 2gnb
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The interplay between metal binding, carbohydrate binding activity, stability and structure of the lectin from Pterocarpus angolensis was investigated. Removal of the metals leads to a more flexible form of the protein with significantly less conformational stability. Crystal structures of this metal-free form show significant structural rearrangements, although some structural features that allow the binding of sugars are retained. We propose that substitution of an asparagine residue at the start of the C-terminal beta-strand of the legume lectin monomer hinders the trans-isomerization of the cis-peptide bond upon demetallization and constitutes an intramolecular switch governing the isomer state of the non-proline bond and ultimately the lectin phenotype.

Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin.,Garcia-Pino A, Buts L, Wyns L, Loris R J Mol Biol. 2006 Aug 4;361(1):153-67. Epub 2006 Jun 21. PMID:16824540^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Garcia-Pino A, Buts L, Wyns L, Loris R. Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin. J Mol Biol. 2006 Aug 4;361(1):153-67. Epub 2006 Jun 21. PMID:16824540 doi:http://dx.doi.org/10.1016/j.jmb.2006.06.006

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gmp"

@@ Line 1: / Line 1: @@
 ==Metal-free (apo) P. angolensis seed lectin in complex with GlcNAC-beta(1-2)Man==
 <StructureSection load='2gmp' size='340' side='right' caption='[[2gmp]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2gmp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pterocarpus_angolensis Pterocarpus angolensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GMP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2gmp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bleedwood_tree Bleedwood tree]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GMP FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ukg|1ukg]], [[2gme|2gme]], [[2gmm|2gmm]], [[2gn3|2gn3]], [[2gnb|2gnb]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gmp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gmp OCA], [http://pdbe.org/2gmp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gmp RCSB], [http://www.ebi.ac.uk/pdbsum/2gmp PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gmp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gmp OCA], [http://pdbe.org/2gmp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gmp RCSB], [http://www.ebi.ac.uk/pdbsum/2gmp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2gmp ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Pterocarpus angolensis]]
+[[Category: Bleedwood tree]]
 [[Category: Buts, L]]
 [[Category: Garcia-Pino, A]]

2gmp

From Proteopedia

Revision as of 07:56, 18 October 2017

Metal-free (apo) P. angolensis seed lectin in complex with GlcNAC-beta(1-2)Man

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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