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2gx9

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Revision as of 08:02, 18 October 2017

X-ray strucutre of influenza virus NS1 effector domain

Structural highlights

2gx9 is a 2 chain structure with sequence from Influenza virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Gene:	Segment 8 - NS1 (Influenza virus)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NS1_I34A1] Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism (By similarity). Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors like IRF3 and IRF7. Prevents human EIF2AK2/PKR activation, either by binding double-strand RNA, or by interacting directly with EIF2AK2/PKR. This function may be important at the very beginning of the infection, when NS1 is mainly present in the cytoplasm. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells (By similarity).

Publication Abstract from PubMed

The nonstructural protein NS1 of influenza virus is an antagonist of host immune responses and is implicated in virulence. It has two domains, an N-terminal double-stranded RNA-binding domain (RBD) and an effector domain crucial for RBD function, for nuclear export and for sequestering messenger RNA-processing proteins. Here we present the crystallographic structure of the effector domain, which has a novel fold and suggests mechanisms for increased virulence in H5N1 strains.

X-ray structure of influenza virus NS1 effector domain.,Bornholdt ZA, Prasad BV Nat Struct Mol Biol. 2006 Jun;13(6):559-60. Epub 2006 May 21. PMID:16715094^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bornholdt ZA, Prasad BV. X-ray structure of influenza virus NS1 effector domain. Nat Struct Mol Biol. 2006 Jun;13(6):559-60. Epub 2006 May 21. PMID:16715094 doi:nsmb1099

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gx9"

@@ Line 1: / Line 1: @@
 ==X-ray strucutre of influenza virus NS1 effector domain==
 <StructureSection load='2gx9' size='340' side='right' caption='[[2gx9]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Segment 8 - NS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11309 Influenza virus])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gx9 OCA], [http://pdbe.org/2gx9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gx9 RCSB], [http://www.ebi.ac.uk/pdbsum/2gx9 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gx9 OCA], [http://pdbe.org/2gx9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gx9 RCSB], [http://www.ebi.ac.uk/pdbsum/2gx9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2gx9 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 19: / Line 20: @@
 </div>
 <div class="pdbe-citations 2gx9" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Nonstructural protein|Nonstructural protein]]
 == References ==
 <references/>

2gx9

From Proteopedia

Revision as of 08:02, 18 October 2017

X-ray strucutre of influenza virus NS1 effector domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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