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2h9a

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Revision as of 08:08, 18 October 2017

Corrinoid Iron-Sulfur Protein

Structural highlights

2h9a is a 2 chain structure with sequence from Atcc baa-161. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The cobalt- and iron-containing corrinoid iron-sulfur protein (CoFeSP) is functional in the acetyl-CoA (Ljungdahl-Wood) pathway of autotrophic carbon fixation in various bacteria and archaea, where it is essential for the biosynthesis of acetyl-CoA. CoFeSP acts in two methylation reactions: the transfer of a methyl group from methyltransferase (MeTr)-bound methyltetrahydrofolate to the cob(I)amide of CoFeSP and the transfer of the methyl group of methyl-cob(III)amide to the reduced Ni-Ni-[4Fe-4S] active site cluster A of acetyl-CoA synthase (ACS). We have solved the crystal structure of as-isolated CoFeSP(Ch) from the CO-oxidizing hydrogenogenic bacterium Carboxydothermus hydrogenoformans at 1.9-A resolution. The heterodimeric protein consists of two tightly interacting subunits with pseudo-twofold symmetry. The large CfsA subunit comprises three domains, of which the N-terminal domain binds the [4Fe-4S] cluster, the middle domain is a (betaalpha)(8)-barrel, and the C-terminal domain shows an open fold and binds Cobeta-aqua-(5,6-dimethylbenzimidazolylcobamide) in a "base-off" state without a protein ligand at the cobalt ion. The small CfsB subunit also displays a (betaalpha)(8)-barrel fold and interacts with the upper side of the corrin macrocycle. Structure-based alignments show that both (betaalpha)(8)-barrel domains are related to the MeTr in the acetyl-CoA pathway and to the folate domain of methionine synthase. We suggest that the C-terminal domain of the large subunit is the mobile element that allows the necessary interaction of CoFeSP(Ch) with the active site of ACS(Ch) and the methyltetrahydrofolate carrying MeTr. The conformation in the crystal structure shields the two open coordinations of cobalt and likely represents a resting state.

Structural insights into methyltransfer reactions of a corrinoid iron-sulfur protein involved in acetyl-CoA synthesis.,Svetlitchnaia T, Svetlitchnyi V, Meyer O, Dobbek H Proc Natl Acad Sci U S A. 2006 Sep 26;103(39):14331-6. Epub 2006 Sep 18. PMID:16983091^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Svetlitchnaia T, Svetlitchnyi V, Meyer O, Dobbek H. Structural insights into methyltransfer reactions of a corrinoid iron-sulfur protein involved in acetyl-CoA synthesis. Proc Natl Acad Sci U S A. 2006 Sep 26;103(39):14331-6. Epub 2006 Sep 18. PMID:16983091

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2h9a"

Categories: Atcc baa-161 | Dobbek, H | Beta-alpha-barrel | Heterodimer | Oxidoreductase

@@ Line 1: / Line 1: @@
 ==Corrinoid Iron-Sulfur Protein==
 <StructureSection load='2h9a' size='340' side='right' caption='[[2h9a]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2h9a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Carboxydothermus_hydrogenoformans Carboxydothermus hydrogenoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H9A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H9A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2h9a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_baa-161 Atcc baa-161]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H9A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H9A FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h9a OCA], [http://pdbe.org/2h9a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h9a RCSB], [http://www.ebi.ac.uk/pdbsum/2h9a PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h9a OCA], [http://pdbe.org/2h9a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h9a RCSB], [http://www.ebi.ac.uk/pdbsum/2h9a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2h9a ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 14: / Line 15: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h9a ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 28: / Line 29: @@
 ==See Also==
 *[[Acetyl-CoA synthase|Acetyl-CoA synthase]]
-*[[Carbon monoxide dehydrogenase|Carbon monoxide dehydrogenase]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Carboxydothermus hydrogenoformans]]
+[[Category: Atcc baa-161]]
 [[Category: Dobbek, H]]
 [[Category: Beta-alpha-barrel]]
 [[Category: Heterodimer]]
 [[Category: Oxidoreductase]]

2h9a

From Proteopedia

Revision as of 08:08, 18 October 2017

Corrinoid Iron-Sulfur Protein

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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