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2i5w

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Revision as of 08:23, 18 October 2017

Structure of hOGG1 crosslinked to DNA sampling a normal G adjacent to an oxoG

Structural highlights

2i5w is a 3 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Related:	1yqk, 1ebm, 1ko9
Gene:	OGG1, MMH, MUTM, OGH1 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[OGG1_HUMAN] Defects in OGG1 may be a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.

Function

[OGG1_HUMAN] DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

How DNA glycosylases search through millions of base pairs and discriminate between rare sites of damage and otherwise undamaged bases is poorly understood. Even less understood are the details of the structural states arising from DNA glycosylases interacting with undamaged DNA. Recognizing the mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoguanine, oxoG) represents an especially formidable challenge, because this oxidized nucleobase differs by only two atoms from its normal counterpart, guanine (G), and buried in the structure of naked B-form DNA, oxoG and G are practically indistinguishable from each other. We have used disulfide cross-linking technology to capture a human oxoG repair protein, 8-oxoguanine DNA glycosylase I (hOGG1) sampling an undamaged G:C base pair located adjacent to an oxoG:C base pair in DNA. The x-ray structure of the trapped complex reveals that the presence of the 8-oxoG drastically changes the local conformation of the extruded G. The extruded but intrahelical state of the G in this structure offers a view of an early intermediate in the base-extrusion pathway.

A nucleobase lesion remodels the interaction of its normal neighbor in a DNA glycosylase complex.,Banerjee A, Verdine GL Proc Natl Acad Sci U S A. 2006 Oct 10;103(41):15020-5. Epub 2006 Oct 2. PMID:17015827^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Banerjee A, Verdine GL. A nucleobase lesion remodels the interaction of its normal neighbor in a DNA glycosylase complex. Proc Natl Acad Sci U S A. 2006 Oct 10;103(41):15020-5. Epub 2006 Oct 2. PMID:17015827

1 Structural highlights
2 Disease
3 Function
4 Evolutionary Conservation
5 Publication Abstract from PubMed
6 See Also
7 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2i5w"

@@ Line 1: / Line 1: @@
 ==Structure of hOGG1 crosslinked to DNA sampling a normal G adjacent to an oxoG==
 <StructureSection load='2i5w' size='340' side='right' caption='[[2i5w]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
@@ Line 7: / Line 8: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1yqk|1yqk]], [[1ebm|1ebm]], [[1ko9|1ko9]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">OGG1, MMH, MUTM, OGH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5w OCA], [http://pdbe.org/2i5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i5w RCSB], [http://www.ebi.ac.uk/pdbsum/2i5w PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5w OCA], [http://pdbe.org/2i5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i5w RCSB], [http://www.ebi.ac.uk/pdbsum/2i5w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i5w ProSAT]</span></td></tr>
 </table>
 == Disease ==

2i5w

From Proteopedia

Revision as of 08:23, 18 October 2017

Structure of hOGG1 crosslinked to DNA sampling a normal G adjacent to an oxoG

Structural highlights

Disease

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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