2inc

From Proteopedia

(Difference between revisions)

Revision as of 08:32, 18 October 2017

Native Toluene/o-xylene Monooxygenase Hydroxylase X-ray Crystal Structure

Structural highlights

2inc is a 3 chain structure with sequence from "achromobacter_sewerinii"_bergey_et_al._1923 "achromobacter sewerinii" bergey et al. 1923. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	1t0q
Gene:	touA ("Achromobacter sewerinii" Bergey et al. 1923), touE ("Achromobacter sewerinii" Bergey et al. 1923), touB ("Achromobacter sewerinii" Bergey et al. 1923)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We report the X-ray crystal structures of native and manganese(II)-reconstituted toluene/o-xylene monooxygenase hydroxylase (ToMOH) from Pseudomonas stutzeri OX1 to 1.85 and 2.20 A resolution, respectively. The structures reveal that reduction of the dimetallic active site is accompanied by a carboxylate shift and alteration of the coordination environment for dioxygen binding and activation. A rotamer shift in a strategically placed asparagine 202 accompanies dimetallic center reduction and is proposed to influence protein component interactions. This rotamer shift is conserved between ToMOH and the corresponding residue in methane monooxygenase hydroxylase (MMOH). Previously unidentified hydrophobic pockets similar to those present in MMOH are assigned.

X-ray crystal structures of manganese(II)-reconstituted and native toluene/o-xylene monooxygenase hydroxylase reveal rotamer shifts in conserved residues and an enhanced view of the protein interior.,McCormick MS, Sazinsky MH, Condon KL, Lippard SJ J Am Chem Soc. 2006 Nov 29;128(47):15108-10. PMID:17117860^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ McCormick MS, Sazinsky MH, Condon KL, Lippard SJ. X-ray crystal structures of manganese(II)-reconstituted and native toluene/o-xylene monooxygenase hydroxylase reveal rotamer shifts in conserved residues and an enhanced view of the protein interior. J Am Chem Soc. 2006 Nov 29;128(47):15108-10. PMID:17117860 doi:10.1021/ja064837r

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2inc"

@@ Line 1: / Line 1: @@
 ==Native Toluene/o-xylene Monooxygenase Hydroxylase X-ray Crystal Structure==
 <StructureSection load='2inc' size='340' side='right' caption='[[2inc]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1t0q|1t0q]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">touA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), touE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), touB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2inc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2inc OCA], [http://pdbe.org/2inc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2inc RCSB], [http://www.ebi.ac.uk/pdbsum/2inc PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2inc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2inc OCA], [http://pdbe.org/2inc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2inc RCSB], [http://www.ebi.ac.uk/pdbsum/2inc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2inc ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==

2inc

From Proteopedia

Revision as of 08:32, 18 October 2017

Native Toluene/o-xylene Monooxygenase Hydroxylase X-ray Crystal Structure

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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