2iou

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Revision as of 08:33, 18 October 2017

Major Tropism Determinant P1 (Mtd-P1) Variant Complexed with Bordetella brochiseptica Virulence Factor Pertactin extracellular domain (Prn-E).

Structural highlights

2iou is a 8 chain structure with sequence from Alcaligenes bronchisepticus and Bpbpp. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1yu0, 1dab
Gene:	Mtd (BPBPP), Prn (Alcaligenes bronchisepticus)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[Q775D6_BPBPP] Tail fiber protein located at the distal ends of the fibers that binds to the adhesion receptors on the host surface, thereby determining the host range. The phage can alter its tropism by modifying this protein. Variants are expressed through a diversity-generating retroelement (DGR) that creates mutant copies of a template repeat and replaces the end of the tail fiber receptor-binding protein with these sequences, thus changing the host range. Milliards of variants of the fiber receptor-binding protein can be created with this system.^[1] [PERT_BORBR] Agglutinogen that binds to eukaryotic cells; a process mediated by the R-G-D sequence. Pertactin may have a role in bacterial adhesion, and thus play a role in virulence. May contribute to the disease state of whooping cough.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Diversity-generating retroelements (DGRs) recognize novel ligands through massive protein sequence variation, a property shared uniquely with the adaptive immune response. Little is known about how recognition is achieved by DGR variable proteins. Here, we present the structure of the Bordetella bacteriophage DGR variable protein major tropism determinant (Mtd) bound to the receptor pertactin, revealing remarkable adaptability in the static binding sites of Mtd. Despite large dissimilarities in ligand binding mode, principles underlying selective recognition were strikingly conserved between Mtd and immunoreceptors. Central to this was the differential amplification of binding strengths by avidity (i.e., multivalency), which not only relaxed the demand for optimal complementarity between Mtd and pertactin but also enhanced distinctions among binding events to provide selectivity. A quantitatively similar balance between complementarity and avidity was observed for Bordetella bacteriophage DGR as occurs in the immune system, suggesting that variable repertoires operate under a narrow set of conditions to recognize novel ligands.

Selective ligand recognition by a diversity-generating retroelement variable protein.,Miller JL, Le Coq J, Hodes A, Barbalat R, Miller JF, Ghosh P PLoS Biol. 2008 Jun 3;6(6):e131. PMID:18532877^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Doulatov S, Hodes A, Dai L, Mandhana N, Liu M, Deora R, Simons RW, Zimmerly S, Miller JF. Tropism switching in Bordetella bacteriophage defines a family of diversity-generating retroelements. Nature. 2004 Sep 23;431(7007):476-81. PMID:15386016 doi:http://dx.doi.org/10.1038/nature02833
↑ Miller JL, Le Coq J, Hodes A, Barbalat R, Miller JF, Ghosh P. Selective ligand recognition by a diversity-generating retroelement variable protein. PLoS Biol. 2008 Jun 3;6(6):e131. PMID:18532877 doi:10.1371/journal.pbio.0060131

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2iou"

@@ Line 1: / Line 1: @@
 ==Major Tropism Determinant P1 (Mtd-P1) Variant Complexed with Bordetella brochiseptica Virulence Factor Pertactin extracellular domain (Prn-E).==
 <StructureSection load='2iou' size='340' side='right' caption='[[2iou]], [[Resolution|resolution]] 3.16&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1yu0|1yu0]], [[1dab|1dab]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mtd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=194699 BPBPP]), Prn ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=257310 Alcaligenes bronchisepticus])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iou OCA], [http://pdbe.org/2iou PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iou RCSB], [http://www.ebi.ac.uk/pdbsum/2iou PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iou OCA], [http://pdbe.org/2iou PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iou RCSB], [http://www.ebi.ac.uk/pdbsum/2iou PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2iou ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 29: / Line 30: @@
 </div>
 <div class="pdbe-citations 2iou" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Pertussis toxin|Pertussis toxin]]
 == References ==
 <references/>

2iou

From Proteopedia

Revision as of 08:33, 18 October 2017

Major Tropism Determinant P1 (Mtd-P1) Variant Complexed with Bordetella brochiseptica Virulence Factor Pertactin extracellular domain (Prn-E).

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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