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2npg

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Revision as of 08:38, 18 October 2017

An unusual twin-His arrangement in the pore of ammonia channels is essential for substrate conductance

Structural highlights

2npg is a 1 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	1xqf, 1xqe, 1u7g, 2nmr, 2nop, 2now, 2npc, 2npd, 2npe, 2npj, 2npk
Gene:	amtB ("Bacillus coli" Migula 1895)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[AMTB_ECOLI] Involved in the uptake of ammonia.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Amt proteins constitute a class of ubiquitous integral membrane proteins that mediate movement of ammonium across cell membranes. They are homotrimers, in which each subunit contains a narrow pore through which substrate transport occurs. Two conserved histidine residues in the pore have been proposed to be necessary for ammonia conductance. By analyzing 14 engineered polar and non-polar variants of these histidines, in Escherichia coli AmtB, we show that both histidines are absolutely required for optimum substrate conductance. Crystal structures of variants confirm that substitution of the histidine residues does not affect AmtB structure. In a subgroup of Amt proteins, found only in fungi, one of the histidines is replaced by glutamate. The equivalent substitution in E. coli AmtB is partially active, and the structure of this variant suggests that the glutamate side chain can make similar interactions to those made by histidine.

An unusual twin-his arrangement in the pore of ammonia channels is essential for substrate conductance.,Javelle A, Lupo D, Zheng L, Li XD, Winkler FK, Merrick M J Biol Chem. 2006 Dec 22;281(51):39492-8. Epub 2006 Oct 12. PMID:17040913^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Javelle A, Lupo D, Zheng L, Li XD, Winkler FK, Merrick M. An unusual twin-his arrangement in the pore of ammonia channels is essential for substrate conductance. J Biol Chem. 2006 Dec 22;281(51):39492-8. Epub 2006 Oct 12. PMID:17040913 doi:10.1074/jbc.M608325200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2npg"

@@ Line 1: / Line 1: @@
 ==An unusual twin-His arrangement in the pore of ammonia channels is essential for substrate conductance==
 <StructureSection load='2npg' size='340' side='right' caption='[[2npg]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1xqf|1xqf]], [[1xqe|1xqe]], [[1u7g|1u7g]], [[2nmr|2nmr]], [[2nop|2nop]], [[2now|2now]], [[2npc|2npc]], [[2npd|2npd]], [[2npe|2npe]], [[2npj|2npj]], [[2npk|2npk]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">amtB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2npg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npg OCA], [http://pdbe.org/2npg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2npg RCSB], [http://www.ebi.ac.uk/pdbsum/2npg PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2npg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npg OCA], [http://pdbe.org/2npg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2npg RCSB], [http://www.ebi.ac.uk/pdbsum/2npg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2npg ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2npg ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 29: / Line 30: @@
 </div>
 <div class="pdbe-citations 2npg" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Ion channels|Ion channels]]
 == References ==
 <references/>

2npg

From Proteopedia

Revision as of 08:38, 18 October 2017

An unusual twin-His arrangement in the pore of ammonia channels is essential for substrate conductance

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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