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2ond

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Revision as of 09:04, 18 October 2017

Crystal Structure of the HAT-C domain of murine CstF-77

Structural highlights

2ond is a 2 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Gene:	Cstf3 (LK3 transgenic mice)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CSTF3_MOUSE] One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cleavage stimulation factor (CstF) is a heterotrimeric protein complex essential for polyadenylation of mRNA precursors. The 77 kDa subunit, CstF-77, is known to mediate interactions with the other two subunits of CstF as well as with other components of the polyadenylation machinery. We report here the crystal structure of the HAT (half a TPR) domain of murine CstF-77, as well as its C-terminal subdomain. Structural and biochemical studies show that the HAT domain consists of two subdomains, HAT-N and HAT-C domains, with drastically different orientations of their helical motifs. The structures reveal a highly elongated dimer, spanning 165 A, with the dimerization mediated by the HAT-C domain. Light-scattering studies, yeast two-hybrid assays, and analytical ultracentrifugation measurements confirm this self-association. The mode of dimerization and the relative arrangement of the HAT-N and HAT-C domains are unique to CstF-77. Our data support a role for CstF dimerization in pre-mRNA 3' end processing.

Crystal structure of murine CstF-77: dimeric association and implications for polyadenylation of mRNA precursors.,Bai Y, Auperin TC, Chou CY, Chang GG, Manley JL, Tong L Mol Cell. 2007 Mar 23;25(6):863-75. PMID:17386263^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bai Y, Auperin TC, Chou CY, Chang GG, Manley JL, Tong L. Crystal structure of murine CstF-77: dimeric association and implications for polyadenylation of mRNA precursors. Mol Cell. 2007 Mar 23;25(6):863-75. PMID:17386263 doi:10.1016/j.molcel.2007.01.034

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ond"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the HAT-C domain of murine CstF-77==
 <StructureSection load='2ond' size='340' side='right' caption='[[2ond]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cstf3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ond FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ond OCA], [http://pdbe.org/2ond PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ond RCSB], [http://www.ebi.ac.uk/pdbsum/2ond PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ond FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ond OCA], [http://pdbe.org/2ond PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ond RCSB], [http://www.ebi.ac.uk/pdbsum/2ond PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ond ProSAT]</span></td></tr>
 </table>
 == Function ==

2ond

From Proteopedia

Revision as of 09:04, 18 October 2017

Crystal Structure of the HAT-C domain of murine CstF-77

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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