Mediator
From Proteopedia
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<StructureSection load='1yke' size='350' side='right' caption='Yeast mediator head module Med7 (grey) and Med21 (green) (PDB entry [[1yke]])' scene=''> | <StructureSection load='1yke' size='350' side='right' caption='Yeast mediator head module Med7 (grey) and Med21 (green) (PDB entry [[1yke]])' scene=''> | ||
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| - | <StructureSection load='3dab' size='350' side='right' caption='Human MDM4 p53-binding domain (grey) complex with p53 peptide (green) (PDB entry [[3dab]])' scene=''> | ||
== Mediator == | == Mediator == | ||
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The <scene name='Sandbox_Reserved_387/Head_module/1'>head module</scene> of mediator from ''S.cerevisiae'' is made of seven subunits which are Med22(Srb6), Med20(Srb2), Med18(Srb5), Med17(Srb4), Med11, Med6, and Med8.<ref name="nature" /> Of these subunits, Med11, Med17(Srb4), and Med22(Srb6) make the mini-head of the head module. The subunits of the core-head plus the subunits of the mini-head are what make up full head structure. The structure shows the three domains: the neck, which is shown by pink helices, the fixed jaw, which is shown by mostly pink helices and a few yellow beta sheets, and the movable jaw, which is shown by mostly yellow beta sheets and a few pink helices. The '''tail module''' of yeast mediator is made up of Med2, Med3, Med14, Med15 and Med16. The '''middle module''' of yeast mediator is made up of Med1, Med4, Med5, Med7, Med10, Med12, Med13, Med19, Med21, Med26, Med31, CycC and Cdk8. | The <scene name='Sandbox_Reserved_387/Head_module/1'>head module</scene> of mediator from ''S.cerevisiae'' is made of seven subunits which are Med22(Srb6), Med20(Srb2), Med18(Srb5), Med17(Srb4), Med11, Med6, and Med8.<ref name="nature" /> Of these subunits, Med11, Med17(Srb4), and Med22(Srb6) make the mini-head of the head module. The subunits of the core-head plus the subunits of the mini-head are what make up full head structure. The structure shows the three domains: the neck, which is shown by pink helices, the fixed jaw, which is shown by mostly pink helices and a few yellow beta sheets, and the movable jaw, which is shown by mostly yellow beta sheets and a few pink helices. The '''tail module''' of yeast mediator is made up of Med2, Med3, Med14, Med15 and Med16. The '''middle module''' of yeast mediator is made up of Med1, Med4, Med5, Med7, Med10, Med12, Med13, Med19, Med21, Med26, Med31, CycC and Cdk8. | ||
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==3D structures of mediator== | ==3D structures of mediator== | ||
Revision as of 16:25, 20 October 2017
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3D structures of mediator
3rj1 – Med6 + Med8 + Med11 + Med17 + Med18 + Med20 + Med22 - yeast
1yke, 1ykh – Med7 + Med21
3fbi, 3fbn – Med7 N terminal + Med31
3c0t - Med8 C terminal + Med18
2hzs – Med8 C terminal + Med18 + Med20
3r84 - Med11 + Med22 (mutant)
2lpb - Med15 + general control protein GCN4
2hzm – Med18 + Med20
2l23, 2l6u, 2ky6, 2xnf – Med25 activator-interacting domain - NMR
References
- ↑ Imasaki T, Calero G, Cai G, Tsai KL, Yamada K, Cardelli F, Erdjument-Bromage H, Tempst P, Berger I, Kornberg GL, Asturias FJ, Kornberg RD, Takagi Y. Architecture of the Mediator head module. Nature. 2011 Jul 3;475(7355):240-3. doi: 10.1038/nature10162. PMID:21725323 doi:10.1038/nature10162
- ↑ Taatjes DJ. The human Mediator complex: a versatile, genome-wide regulator of transcription. Trends Biochem Sci. 2010 Jun;35(6):315-22. Epub 2010 Mar 17. PMID:20299225 doi:10.1016/j.tibs.2010.02.004
- ↑ 3.0 3.1 Imasaki T, Calero G, Cai G, Tsai KL, Yamada K, Cardelli F, Erdjument-Bromage H, Tempst P, Berger I, Kornberg GL, Asturias FJ, Kornberg RD, Takagi Y. Architecture of the Mediator head module. Nature. 2011 Jul 3;475(7355):240-3. doi: 10.1038/nature10162. PMID:21725323 doi:10.1038/nature10162
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Joel L. Sussman, Bradley Altier, Alexander Berchansky
