1z4n
From Proteopedia
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|PDB= 1z4n |SIZE=350|CAPTION= <scene name='initialview01'>1z4n</scene>, resolution 1.97Å | |PDB= 1z4n |SIZE=350|CAPTION= <scene name='initialview01'>1z4n</scene>, resolution 1.97Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GL1:1-O-PHOSPHONO-BETA-L-ALLOPYRANOSE'>GL1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Beta-phosphoglucomutase Beta-phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.6 5.4.2.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-phosphoglucomutase Beta-phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.6 5.4.2.6] </span> |
|GENE= pgmB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 Lactococcus lactis]) | |GENE= pgmB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 Lactococcus lactis]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1z4o|1Z4O]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z4n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z4n OCA], [http://www.ebi.ac.uk/pdbsum/1z4n PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z4n RCSB]</span> | ||
}} | }} | ||
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[[Category: Tremblay, L W.]] | [[Category: Tremblay, L W.]] | ||
[[Category: Zhang, G.]] | [[Category: Zhang, G.]] | ||
| - | [[Category: GL1]] | ||
| - | [[Category: MG]] | ||
[[Category: beta-phosphoglucomutase]] | [[Category: beta-phosphoglucomutase]] | ||
[[Category: isomerase]] | [[Category: isomerase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:29:02 2008'' |
Revision as of 22:29, 30 March 2008
| |||||||
| , resolution 1.97Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | pgmB (Lactococcus lactis) | ||||||
| Activity: | Beta-phosphoglucomutase, with EC number 5.4.2.6 | ||||||
| Related: | 1Z4O
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate cocrystallized with Fluoride
Overview
This communication reports the X-ray crystal structure of the alpha-d-galactose-1-phosphate complex with that of Lactococcus lactis beta-phosphoglucomutase (beta-PGM) crystallized in the presence of Mg2+ cofactor and the enzyme-to-phosphorus ratio determined by protein and phosphate analyses of the crystalline complex. The 1:1 ratio determined for this complex was compared to the 1:2 ratio determined for the crystals of beta-PGM grown in the presence of substrate and Mg2+ cofactor. This result verifies the published structure assignment of this latter complex as the phosphorane adduct formed by covalent bonding between the active site Asp8 carboxylate to the C(1)phosphorus of the beta-glucose 1,6-bisphosphate ligand and rules out the proposal of a beta-PGM-glucose-6-phosphate-1-MgF3- complex.
About this Structure
1Z4N is a Single protein structure of sequence from Lactococcus lactis. Full crystallographic information is available from OCA.
Reference
Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase., Tremblay LW, Zhang G, Dai J, Dunaway-Mariano D, Allen KN, J Am Chem Soc. 2005 Apr 20;127(15):5298-9. PMID:15826149
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