1z5u
From Proteopedia
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|PDB= 1z5u |SIZE=350|CAPTION= <scene name='initialview01'>1z5u</scene>, resolution 2.3Å | |PDB= 1z5u |SIZE=350|CAPTION= <scene name='initialview01'>1z5u</scene>, resolution 2.3Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=CMP:ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE'>CMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span> |
|GENE= aphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium]) | |GENE= aphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1z5g|1Z5G]], [[1z88|1Z88]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z5u OCA], [http://www.ebi.ac.uk/pdbsum/1z5u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z5u RCSB]</span> | ||
}} | }} | ||
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[[Category: Kumar, V.]] | [[Category: Kumar, V.]] | ||
[[Category: Makde, R D.]] | [[Category: Makde, R D.]] | ||
| - | [[Category: CMP]] | ||
| - | [[Category: MG]] | ||
[[Category: class-b bacterial acid phosphatase]] | [[Category: class-b bacterial acid phosphatase]] | ||
[[Category: cyclic-amp complex of apha protein]] | [[Category: cyclic-amp complex of apha protein]] | ||
[[Category: metalloenzyme]] | [[Category: metalloenzyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:29:32 2008'' |
Revision as of 22:29, 30 March 2008
| |||||||
| , resolution 2.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | aphA (Salmonella typhimurium) | ||||||
| Activity: | Acid phosphatase, with EC number 3.1.3.2 | ||||||
| Related: | 1Z5G, 1Z88
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of S. typhimurium AphA complexed with cyclic-AMP
Overview
The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA protein was purified to homogeneity. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent content of approximately 54%. The crystals are composed of biologically active AphA molecules.
About this Structure
1Z5U is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium., Makde RD, Kumar V, Gupta GD, Jasti J, Singh TP, Mahajan SK, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1849-52. Epub 2003, Sep 19. PMID:14501135
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