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3c21

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Revision as of 08:16, 25 October 2017

Structure of a bacterial DNA damage sensor protein with reaction product

Structural highlights

3c21 is a 2 chain structure with sequence from Atcc 43589. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3c1y, 3c1z, 3c23
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DISA_THEMA] Participates in a DNA-damage check-point. DisA forms globular foci that rapidly scan along the chromosomes searching for lesions (By similarity).^[1] Has also diadenylate cyclase activity, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP likely acts as a signaling molecule that may couple DNA integrity with a cellular process. Does not convert GTP to c-di-GMP.^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

To reveal mechanisms of DNA damage checkpoint initiation, we structurally and biochemically analyzed DisA, a protein that controls a Bacillus subtilis sporulation checkpoint in response to DNA double-strand breaks. We find that DisA forms a large octamer that consists of an array of an uncharacterized type of nucleotide-binding domain along with two DNA-binding regions related to the Holliday junction recognition protein RuvA. Remarkably, the nucleotide-binding domains possess diadenylate cyclase activity. The resulting cyclic diadenosine phosphate, c-di-AMP, is reminiscent but distinct from c-di-GMP, an emerging prokaryotic regulator of complex cellular processes. Diadenylate cyclase activity is unaffected by linear DNA or DNA ends but strongly suppressed by branched nucleic acids such as Holliday junctions. Our data indicate that DisA signals DNA structures that interfere with chromosome segregation via c-di-AMP. Identification of the diadenylate cyclase domain in other eubacterial and archaeal proteins implies a more general role for c-di-AMP in prokaryotes.

Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates.,Witte G, Hartung S, Buttner K, Hopfner KP Mol Cell. 2008 Apr 25;30(2):167-78. PMID:18439896^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Witte G, Hartung S, Buttner K, Hopfner KP. Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates. Mol Cell. 2008 Apr 25;30(2):167-78. PMID:18439896 doi:10.1016/j.molcel.2008.02.020
↑ Witte G, Hartung S, Buttner K, Hopfner KP. Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates. Mol Cell. 2008 Apr 25;30(2):167-78. PMID:18439896 doi:10.1016/j.molcel.2008.02.020
↑ Witte G, Hartung S, Buttner K, Hopfner KP. Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates. Mol Cell. 2008 Apr 25;30(2):167-78. PMID:18439896 doi:10.1016/j.molcel.2008.02.020

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3c21"

@@ Line 1: / Line 1: @@
 ==Structure of a bacterial DNA damage sensor protein with reaction product==
 <StructureSection load='3c21' size='340' side='right' caption='[[3c21]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2BA:(2R,3R,3AS,5R,7AR,9R,10R,10AS,12R,14AR)-2,9-BIS(6-AMINO-9H-PURIN-9-YL)OCTAHYDRO-2H,7H-DIFURO[3,2-D 3,2-J][1,3,7,9,2,8]TETRAOXADIPHOSPHACYCLODODECINE-3,5,10,12-TETROL+5,12-DIOXIDE'>2BA</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3c1y|3c1y]], [[3c1z|3c1z]], [[3c23|3c23]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c21 OCA], [http://pdbe.org/3c21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c21 RCSB], [http://www.ebi.ac.uk/pdbsum/3c21 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c21 OCA], [http://pdbe.org/3c21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c21 RCSB], [http://www.ebi.ac.uk/pdbsum/3c21 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3c21 ProSAT]</span></td></tr>
 </table>
 == Function ==

3c21

From Proteopedia

Revision as of 08:16, 25 October 2017

Structure of a bacterial DNA damage sensor protein with reaction product

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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