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3d9l

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Revision as of 08:44, 25 October 2017

Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the Carboxy-Terminal Domain of RNA-Polymerase II

Structural highlights

3d9l is a 4 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:	,
Related:	1sz9, 1sza, 2bf0, 3clj, 3d9i, 3d9j, 3d9k, 3d9m, 3d9n, 3d9o, 3d9p
Gene:	RBM16, KIAA1116 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SCAF8_HUMAN] May play a role in mRNA processing.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Concomitant with RNA polymerase II (Pol II) transcription, RNA maturation factors are recruited to the carboxyl-terminal domain (CTD) of Pol II, whose phosphorylation state changes during a transcription cycle. CTD phosphorylation triggers recruitment of functionally different factors involved in RNA processing and transcription termination; most of these factors harbor a conserved CTD interacting domain (CID). Orchestration of factor recruitment is believed to be conducted by CID recognition of distinct phosphorylated forms of the CTD. We show that the human RNA processing factor SCAF8 interacts weakly with the unphosphorylated CTD of Pol II. Upon phosphorylation, affinity for the CTD is increased; however, SCAF8 is promiscuous to the phosphorylation pattern on the CTD. Employing a combined structural and biophysical approach, we were able to distinguish motifs within CIDs that are involved in a generic CTD sequence recognition from items that confer phospho-specificity.

Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the carboxyl-terminal domain of RNA polymerase II.,Becker R, Loll B, Meinhart A J Biol Chem. 2008 Aug 15;283(33):22659-69. Epub 2008 Jun 11. PMID:18550522^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI. Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry. EMBO J. 2000 Dec 1;19(23):6569-81. PMID:11101529 doi:10.1093/emboj/19.23.6569
↑ Becker R, Loll B, Meinhart A. Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the carboxyl-terminal domain of RNA polymerase II. J Biol Chem. 2008 Aug 15;283(33):22659-69. Epub 2008 Jun 11. PMID:18550522 doi:10.1074/jbc.M803540200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3d9l"

@@ Line 1: / Line 1: @@
 ==Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the Carboxy-Terminal Domain of RNA-Polymerase II==
 <StructureSection load='3d9l' size='340' side='right' caption='[[3d9l]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
@@ Line 7: / Line 8: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1sz9|1sz9]], [[1sza|1sza]], [[2bf0|2bf0]], [[3clj|3clj]], [[3d9i|3d9i]], [[3d9j|3d9j]], [[3d9k|3d9k]], [[3d9m|3d9m]], [[3d9n|3d9n]], [[3d9o|3d9o]], [[3d9p|3d9p]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RBM16, KIAA1116 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d9l OCA], [http://pdbe.org/3d9l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d9l RCSB], [http://www.ebi.ac.uk/pdbsum/3d9l PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3d9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d9l OCA], [http://pdbe.org/3d9l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3d9l RCSB], [http://www.ebi.ac.uk/pdbsum/3d9l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3d9l ProSAT]</span></td></tr>
 </table>
 == Function ==

3d9l

From Proteopedia

Revision as of 08:44, 25 October 2017

Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the Carboxy-Terminal Domain of RNA-Polymerase II

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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