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3ejf

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Revision as of 09:12, 25 October 2017

Crystal structure of IBV X-domain at pH 8.5

Structural highlights

3ejf is a 1 chain structure with sequence from Avian infectious bronchitis virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3ejg, 3eke
Gene:	1a (Avian infectious bronchitis virus)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[R1A_IBVB] The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK.[PROSITE-ProRule:PRU00772] The peptide p16 might be involved in the EGF signaling pathway. Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter. Nsp9 is a ssRNA-binding protein.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The polyproteins of coronaviruses are cleaved by viral proteases into at least 15 nonstructural proteins (Nsps). Consisting of five domains, Nsp3 is the largest of these (180-210 kDa). Among these domains, the so-called X-domain is believed to act as ADP-ribose-1-phosphate phosphatase or to bind poly(ADP-ribose). However, here we show that the X-domain of Infectious Bronchitis Virus (strain Beaudette), a Group-3 coronavirus, fails to bind ADP-ribose. This is explained on the basis of the crystal structure of the protein, determined at two different pH values. For comparison, we also describe the crystal structure of the homologous X-domain from Human Coronavirus 229E, a Group-1 coronavirus, which does bind ADP-ribose.

Crystal structures of the X-domains of a Group-1 and a Group-3 coronavirus reveal that ADP-ribose-binding may not be a conserved property.,Piotrowski Y, Hansen G, Boomaars-van der Zanden AL, Snijder EJ, Gorbalenya AE, Hilgenfeld R Protein Sci. 2009 Jan;18(1):6-16. PMID:19177346^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Piotrowski Y, Hansen G, Boomaars-van der Zanden AL, Snijder EJ, Gorbalenya AE, Hilgenfeld R. Crystal structures of the X-domains of a Group-1 and a Group-3 coronavirus reveal that ADP-ribose-binding may not be a conserved property. Protein Sci. 2009 Jan;18(1):6-16. PMID:19177346 doi:10.1002/pro.15

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ejf"

@@ Line 1: / Line 1: @@
 ==Crystal structure of IBV X-domain at pH 8.5==
 <StructureSection load='3ejf' size='340' side='right' caption='[[3ejf]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ejg|3ejg]], [[3eke|3eke]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11120 Avian infectious bronchitis virus])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ejf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ejf OCA], [http://pdbe.org/3ejf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ejf RCSB], [http://www.ebi.ac.uk/pdbsum/3ejf PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ejf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ejf OCA], [http://pdbe.org/3ejf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ejf RCSB], [http://www.ebi.ac.uk/pdbsum/3ejf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ejf ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 42: / Line 43: @@
 [[Category: Macro domain]]
 [[Category: Nsp3]]
+[[Category: Ribosomal frameshifting]]
 [[Category: Rna-binding]]
 [[Category: X-domain]]

3ejf

From Proteopedia

Revision as of 09:12, 25 October 2017

Crystal structure of IBV X-domain at pH 8.5

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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