1zfo
From Proteopedia
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|PDB= 1zfo |SIZE=350|CAPTION= <scene name='initialview01'>1zfo</scene> | |PDB= 1zfo |SIZE=350|CAPTION= <scene name='initialview01'>1zfo</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zfo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zfo OCA], [http://www.ebi.ac.uk/pdbsum/1zfo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zfo RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Otting, G.]] | [[Category: Otting, G.]] | ||
[[Category: Sillard, R.]] | [[Category: Sillard, R.]] | ||
| - | [[Category: ACE]] | ||
| - | [[Category: ZN]] | ||
[[Category: lim domain]] | [[Category: lim domain]] | ||
[[Category: metal-binding protein]] | [[Category: metal-binding protein]] | ||
[[Category: zinc-finger]] | [[Category: zinc-finger]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:35:21 2008'' |
Revision as of 22:35, 30 March 2008
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| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
AMINO-TERMINAL LIM-DOMAIN PEPTIDE OF LASP-1, NMR
Overview
The three-dimensional solution structure of the 1:1 complex between the synthetic peptide ZF-1 and zinc was determined by 1H NMR spectroscopy. The peptide, initially isolated from pig intestines, is identical in sequence to the 30 N-terminal amino acid residues of the human protein Lasp-1 belonging to the LIM domain protein family. The final set of 20 energy-refined NMR conformers has an average rmsd relative to the mean structure of 0.55 A for the backbone atoms of residues 3-30. Calculations without zinc atom constraints unambiguously identified Cys 5, Cys 8, His 26, and Cys 29 as the zinc-coordinating residues. LIM domains consist of two sequential zinc-binding modules and the NMR structure of the ZF-1-zinc complex is the first example of a structure of an isolated module. Comparison with the known structures of the N-terminal zinc-binding modules of both the second LIM domain of chicken CRP and rat CRIP with which ZF-1 shares 50% and 43% sequence identity, respectively, supports the notion that the zinc-binding modules of the LIM domain have a conserved structural motif and identifies local regions of structural diversity. The similarities include conserved zinc-coordinating residues, a rubredoxin knuckle involving Cys 5 and Cys 8, and the coordination of the zinc ion by histidine N delta in contrast to the more usual coordination by N epsilon observed for other zinc-finger domains. The present structure determination of the ZF-1-zinc complex establishes the N-terminal half of a LIM domain as an independent folding unit. The structural similarities of N- and C-terminal zinc-binding modules of the LIM domains, despite limited sequence identity, lead to the proposal of a single zinc-binding motif in LIM domains. The coordinates are available from the Brookhaven protein data bank, entry 1ZFO.
About this Structure
1ZFO is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
Solution structure of a naturally-occurring zinc-peptide complex demonstrates that the N-terminal zinc-binding module of the Lasp-1 LIM domain is an independent folding unit., Hammarstrom A, Berndt KD, Sillard R, Adermann K, Otting G, Biochemistry. 1996 Oct 1;35(39):12723-32. PMID:8841116
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