1zhh
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1zhh |SIZE=350|CAPTION= <scene name='initialview01'>1zhh</scene>, resolution 1.94Å | |PDB= 1zhh |SIZE=350|CAPTION= <scene name='initialview01'>1zhh</scene>, resolution 1.94Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID'>NHE</scene> | + | |LIGAND= <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= luxP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 Vibrio harveyi]), luxQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 Vibrio harveyi]) | |GENE= luxP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 Vibrio harveyi]), luxQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 Vibrio harveyi]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zhh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zhh OCA], [http://www.ebi.ac.uk/pdbsum/1zhh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zhh RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Miller, S T.]] | [[Category: Miller, S T.]] | ||
[[Category: Neiditch, M B.]] | [[Category: Neiditch, M B.]] | ||
| - | [[Category: NHE]] | ||
[[Category: autoinducer-2 (ai-2)]] | [[Category: autoinducer-2 (ai-2)]] | ||
[[Category: per/arnt/simple-minded (pas) fold]] | [[Category: per/arnt/simple-minded (pas) fold]] | ||
| Line 34: | Line 36: | ||
[[Category: sensor kinase]] | [[Category: sensor kinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:36:46 2008'' |
Revision as of 22:36, 30 March 2008
| |||||||
| , resolution 1.94Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | luxP (Vibrio harveyi), luxQ (Vibrio harveyi) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Apo Form of Vibrio Harveyi LUXP Complexed with the Periplasmic Domain of LUXQ
Overview
The extracellular signaling molecule autoinducer-2 (AI-2) mediates quorum-sensing communication in diverse bacterial species. In marine vibrios, binding of AI-2 to the periplasmic receptor LuxP modulates the activity of the inner membrane sensor kinase LuxQ, transducing the AI-2 information into the cytoplasm. Here, we show that Vibrio harveyi LuxP associates with LuxQ in both the presence and absence of AI-2. The 1.9 A X-ray crystal structure of apoLuxP, complexed with the periplasmic domain of LuxQ, reveals that the latter contains two tandem Per/ARNT/Simple-minded (PAS) folds. Thus, although many prokaryotic PAS folds themselves bind ligands, the LuxQ periplasmic PAS folds instead bind LuxP, monitoring its AI-2 occupancy. Mutations that disrupt the apoLuxP:LuxQ interface sensitize V. harveyi to AI-2, implying that AI-2 binding causes the replacement of one set of LuxP:LuxQ contacts with another. These conformational changes switch LuxQ between two opposing enzymatic activities, each of which conveys information to the cytoplasm about the cell density of the surrounding environment.
About this Structure
1ZHH is a Protein complex structure of sequences from Vibrio harveyi. Full crystallographic information is available from OCA.
Reference
Regulation of LuxPQ receptor activity by the quorum-sensing signal autoinducer-2., Neiditch MB, Federle MJ, Miller ST, Bassler BL, Hughson FM, Mol Cell. 2005 May 27;18(5):507-18. PMID:15916958
Page seeded by OCA on Mon Mar 31 01:36:46 2008
