1zlh
From Proteopedia
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|PDB= 1zlh |SIZE=350|CAPTION= <scene name='initialview01'>1zlh</scene>, resolution 1.70Å | |PDB= 1zlh |SIZE=350|CAPTION= <scene name='initialview01'>1zlh</scene>, resolution 1.70Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Carboxypeptidase_A Carboxypeptidase A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.1 3.4.17.1] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxypeptidase_A Carboxypeptidase A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.1 3.4.17.1] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1zli|1ZLI]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zlh OCA], [http://www.ebi.ac.uk/pdbsum/1zlh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zlh RCSB]</span> | ||
}} | }} | ||
| Line 31: | Line 34: | ||
[[Category: Popowicz, G M.]] | [[Category: Popowicz, G M.]] | ||
[[Category: Sommerhoff, C P.]] | [[Category: Sommerhoff, C P.]] | ||
| - | [[Category: ZN]] | ||
[[Category: beta-defensin fold (tci)]] | [[Category: beta-defensin fold (tci)]] | ||
[[Category: eight-stranded twisted beta-sheet surrounded by eight alpha-helices (cpa)]] | [[Category: eight-stranded twisted beta-sheet surrounded by eight alpha-helices (cpa)]] | ||
[[Category: inhibitor-metallocarboxypeptidase complex]] | [[Category: inhibitor-metallocarboxypeptidase complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:38:18 2008'' |
Revision as of 22:38, 30 March 2008
| |||||||
| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Carboxypeptidase A, with EC number 3.4.17.1 | ||||||
| Related: | 1ZLI
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the tick carboxypeptidase inhibitor in complex with bovine carboxypeptidase A
Overview
The tick carboxypeptidase inhibitor (TCI) is a proteinaceous inhibitor of metallo-carboxypeptidases present in the blood-sucking tick Rhipicephalus bursa. The three-dimensional crystal structures of recombinant TCI bound to bovine carboxypeptidase A and to human carboxypeptidase B have been determined and refined at 1.7 A and at 2.0 A resolution, respectively. TCI consists of two domains that are structurally similar despite the low degree of sequence homology. The domains, each consisting of a short alpha-helix followed by a small twisted antiparallel beta-sheet, show a high level of structural homology to proteins of the beta-defensin-fold family. TCI anchors to the surface of mammalian carboxypeptidases in a double-headed manner not previously seen for carboxypeptidase inhibitors: the last three carboxy-terminal amino acid residues interact with the active site of the enzyme in a way that mimics substrate binding, and the N-terminal domain binds to an exosite distinct from the active-site groove. The structures of these complexes should prove valuable in the applications of TCI as a thrombolytic drug and as a basis for the design of novel bivalent carboxypeptidase inhibitors.
About this Structure
1ZLH is a Protein complex structure of sequences from Bos taurus and Rhipicephalus bursa. Full crystallographic information is available from OCA.
Reference
The three-dimensional structures of tick carboxypeptidase inhibitor in complex with A/B carboxypeptidases reveal a novel double-headed binding mode., Arolas JL, Popowicz GM, Lorenzo J, Sommerhoff CP, Huber R, Aviles FX, Holak TA, J Mol Biol. 2005 Jul 15;350(3):489-98. PMID:15961103
Page seeded by OCA on Mon Mar 31 01:38:18 2008
Categories: Bos taurus | Carboxypeptidase A | Protein complex | Rhipicephalus bursa | Arolas, J L. | Aviles, F X. | Holak, T A. | Huber, R. | Lorenzo, J. | Popowicz, G M. | Sommerhoff, C P. | Beta-defensin fold (tci) | Eight-stranded twisted beta-sheet surrounded by eight alpha-helices (cpa) | Inhibitor-metallocarboxypeptidase complex
