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3fk6

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Revision as of 06:43, 1 November 2017

Crystal structure of TetR triple mutant (H64K, S135L, S138I)

Structural highlights

3fk6 is a 2 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2vke, 2o7o, 2vkv, 2tct, 3fk7
Gene:	tetR ("Bacillus coli" Migula 1895)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TETR2_ECOLX] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The bacterial tetracycline transcription regulation system mediated by the tetracycline repressor (TetR) is widely used to study gene expression in prokaryotes and eukaryotes. To study multiple genes in parallel, a triple mutant TetR(K(64)L(135)I(138)) has been engineered that is selectively induced by the synthetic tetracycline derivative 4-de-dimethylamino-anhydrotetracycline (4-ddma-atc) and no longer by tetracycline, the inducer of wild-type TetR. In the present study, we report the crystal structure of TetR(K(64)L(135)I(138)) in the absence and in complex with 4-ddma-atc at resolutions of 2.1 A. Analysis of the structures in light of the available binding data and previously reported TetR complexes allows for a dissection of the origins of selectivity and specificity. In all crystal structures solved to date, the ligand-binding position, as well as the positioning of the residues lining the binding site, is extremely well conserved, irrespective of the chemical nature of the ligand. Selective recognition of 4-ddma-atc is achieved through fine-tuned hydrogen-bonding constraints introduced by the His64-->Lys substitution, as well as a combination of hydrophobic effect and the removal of unfavorable electrostatic interactions through the introduction of Leu135 and Ile138.

Structural origins for selectivity and specificity in an engineered bacterial repressor-inducer pair.,Klieber MA, Scholz O, Lochner S, Gmeiner P, Hillen W, Muller YA FEBS J. 2009 Oct;276(19):5610-21. Epub 2009 Aug 27. PMID:19712110^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Klieber MA, Scholz O, Lochner S, Gmeiner P, Hillen W, Muller YA. Structural origins for selectivity and specificity in an engineered bacterial repressor-inducer pair. FEBS J. 2009 Oct;276(19):5610-21. Epub 2009 Aug 27. PMID:19712110 doi:10.1111/j.1742-4658.2009.07254.x

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3fk6"

@@ Line 1: / Line 1: @@
 ==Crystal structure of TetR triple mutant (H64K, S135L, S138I)==
 <StructureSection load='3fk6' size='340' side='right' caption='[[3fk6]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vke|2vke]], [[2o7o|2o7o]], [[2vkv|2vkv]], [[2tct|2tct]], [[3fk7|3fk7]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tetR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk6 OCA], [http://pdbe.org/3fk6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fk6 RCSB], [http://www.ebi.ac.uk/pdbsum/3fk6 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk6 OCA], [http://pdbe.org/3fk6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fk6 RCSB], [http://www.ebi.ac.uk/pdbsum/3fk6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fk6 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 28: / Line 29: @@
 </div>
 <div class="pdbe-citations 3fk6" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Tetracycline repressor protein|Tetracycline repressor protein]]
 == References ==
 <references/>
@@ Line 53: / Line 51: @@
 [[Category: Transcription]]
 [[Category: Transcription regulation]]
+[[Category: Transposable element]]

3fk6

From Proteopedia

Revision as of 06:43, 1 November 2017

Crystal structure of TetR triple mutant (H64K, S135L, S138I)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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