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3fyl

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Revision as of 06:52, 1 November 2017

GR DNA binding domain:CGT complex

Structural highlights

3fyl is a 4 chain structure with sequence from Buffalo rat. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	Grl, Nr3c1 (Buffalo rat)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GCR_RAT] Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation (By similarity).^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Genes are not simply turned on or off, but instead their expression is fine-tuned to meet the needs of a cell. How genes are modulated so precisely is not well understood. The glucocorticoid receptor (GR) regulates target genes by associating with specific DNA binding sites, the sequences of which differ between genes. Traditionally, these binding sites have been viewed only as docking sites. Using structural, biochemical, and cell-based assays, we show that GR binding sequences, differing by as little as a single base pair, differentially affect GR conformation and regulatory activity. We therefore propose that DNA is a sequence-specific allosteric ligand of GR that tailors the activity of the receptor toward specific target genes.

DNA binding site sequence directs glucocorticoid receptor structure and activity.,Meijsing SH, Pufall MA, So AY, Bates DL, Chen L, Yamamoto KR Science. 2009 Apr 17;324(5925):407-10. PMID:19372434^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hsiao PW, Fryer CJ, Trotter KW, Wang W, Archer TK. BAF60a mediates critical interactions between nuclear receptors and the BRG1 chromatin-remodeling complex for transactivation. Mol Cell Biol. 2003 Sep;23(17):6210-20. PMID:12917342
↑ Meijsing SH, Pufall MA, So AY, Bates DL, Chen L, Yamamoto KR. DNA binding site sequence directs glucocorticoid receptor structure and activity. Science. 2009 Apr 17;324(5925):407-10. PMID:19372434 doi:324/5925/407

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3fyl"

@@ Line 1: / Line 1: @@
 ==GR DNA binding domain:CGT complex==
 <StructureSection load='3fyl' size='340' side='right' caption='[[3fyl]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Grl, Nr3c1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fyl OCA], [http://pdbe.org/3fyl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fyl RCSB], [http://www.ebi.ac.uk/pdbsum/3fyl PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fyl OCA], [http://pdbe.org/3fyl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3fyl RCSB], [http://www.ebi.ac.uk/pdbsum/3fyl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3fyl ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 28: / Line 29: @@
 </div>
 <div class="pdbe-citations 3fyl" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Glucocorticoid receptor|Glucocorticoid receptor]]
 == References ==
 <references/>
@@ Line 42: / Line 40: @@
 [[Category: Alternative initiation]]
 [[Category: Chromatin regulator]]
+[[Category: Cytoplasm]]
 [[Category: Dna-binding]]
 [[Category: Glucocorticoid]]
@@ Line 50: / Line 49: @@
 [[Category: Nucleus]]
 [[Category: Phosphoprotein]]
+[[Category: Polymorphism]]
 [[Category: Receptor]]
 [[Category: Steroid-binding]]
@@ Line 55: / Line 55: @@
 [[Category: Transcription regulation]]
 [[Category: Transcription-dna complex]]
+[[Category: Ubl conjugation]]
+[[Category: Zinc]]
 [[Category: Zinc-finger]]

3fyl

From Proteopedia

Revision as of 06:52, 1 November 2017

GR DNA binding domain:CGT complex

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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