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3g81

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Revision as of 06:59, 1 November 2017

Crystal structure of the trimeric neck and carbohydrate recognition domain of human surfactant protein D in complex with alpha methyl mannoside

Structural highlights

3g81 is a 3 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	2ork, 2os9, 2orj, 2ggx, 2ggu, 2ria, 3g83, 3g84
Gene:	SFTPD, PSPD, SFTP4 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SFTPD_HUMAN] Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Surfactant protein D (SP-D) plays important roles in antiviral host defense. Although SP-D shows a preference for glucose/maltose, the protein also recognizes D-mannose and a variety of mannose-rich microbial ligands. This latter preference prompted an examination of the mechanisms of mannose recognition, particularly as they relate to high-mannose viral glycans. Trimeric neck+carbohydrate recognition domains from human SP-D (hNCRD) preferred alpha1-2 linked dimannose (DM) over the branched trimannose (TM) core, alpha1-3 or alpha1-6 DM, or D-mannose. Previous studies have shown residues flanking the carbohydrate binding site can fine-tune ligand recognition. A mutant with valine at 343 (R343V) showed enhanced binding to mannan relative to wild-type and R343A. No alteration in affinity was observed for D-mannose or for alpha1-3 or alpha1-6 linked DM; however, substantially increased affinity was observed for alpha1-2DM. Both proteins showed efficient recognition of linear and branched sub-domains of high-mannose glycans on carbohydrate microarrays, and R343V showed increased binding to a subset of the oligosaccharides. Crystallographic analysis of an R343V complex with 1,2-DM showed a novel mode of binding. The disaccharide is bound to calcium by the reducing sugar ring, and a stabilizing H-bond is formed between the 2-OH of the non-reducing sugar ring and Arg349. Although hNCRDs show negligible binding to influenza A virus (IAV), R343V showed markedly enhanced viral neutralizing activity. Hydrophobic substitutions for Arg343 selectively blocked binding of a monoclonal antibody (Hyb 246-05) that inhibits IAV binding activity. Our findings demonstrate an extended ligand binding site for mannosylated ligands and the significant contribution of the 343 side chain to specific recognition of multivalent microbial ligands, including high-mannose viral glycans.

Recognition of Mannosylated Ligands and Influenza A Virus by Human SP-D: Contributions of an Extended Site and Residue 343.,Crouch E, Hartshorn K, Horlacher T, McDonald B, Smith K, Cafarella T, Seaton B, Seeberger P, Head J Biochemistry. 2009 Feb 27. PMID:19249874^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Crouch E, Hartshorn K, Horlacher T, McDonald B, Smith K, Cafarella T, Seaton B, Seeberger P, Head J. Recognition of Mannosylated Ligands and Influenza A Virus by Human SP-D: Contributions of an Extended Site and Residue 343. Biochemistry. 2009 Feb 27. PMID:19249874 doi:10.1021/bi8022703

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3g81"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the trimeric neck and carbohydrate recognition domain of human surfactant protein D in complex with alpha methyl mannoside==
 <StructureSection load='3g81' size='340' side='right' caption='[[3g81]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ork|2ork]], [[2os9|2os9]], [[2orj|2orj]], [[2ggx|2ggx]], [[2ggu|2ggu]], [[2ria|2ria]], [[3g83|3g83]], [[3g84|3g84]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SFTPD, PSPD, SFTP4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3g81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g81 OCA], [http://pdbe.org/3g81 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3g81 RCSB], [http://www.ebi.ac.uk/pdbsum/3g81 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3g81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g81 OCA], [http://pdbe.org/3g81 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3g81 RCSB], [http://www.ebi.ac.uk/pdbsum/3g81 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3g81 ProSAT]</span></td></tr>
 </table>
 == Function ==

3g81

From Proteopedia

Revision as of 06:59, 1 November 2017

Crystal structure of the trimeric neck and carbohydrate recognition domain of human surfactant protein D in complex with alpha methyl mannoside

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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