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3gqk

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Revision as of 07:10, 1 November 2017

Crystal Structure of the Bacteriophage phi29 gene product 12 C-terminal fragment in complex with ATP

Structural highlights

3gqk is a 1 chain structure with sequence from Bpph2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	3gq7, 3gq8, 3gq9, 3gqa, 3gqh, 3gqn
Gene:	12, gene product 12 (BPPH2)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The tailed bacteriophage phi29 has 12 "appendages" (gene product 12, gp12) attached to its neck region that participate in host cell recognition and entry. In the cell, monomeric gp12 undergoes proteolytic processing that releases the C-terminal domain during assembly into trimers. We report here crystal structures of the protein before and after catalytic processing and show that the C-terminal domain of gp12 is an "autochaperone" that aids trimerization. We also show that autocleavage of the C-terminal domain is a posttrimerization event that is followed by a unique ATP-dependent release. The posttranslationally modified N-terminal part has three domains that function to attach the appendages to the phage, digest the cell wall teichoic acids, and bind irreversibly to the host, respectively. Structural and sequence comparisons suggest that some eukaryotic and bacterial viruses as well as bacterial adhesins might have a similar maturation mechanism as is performed by phi29 gp12 for Bacillus subtilis.

Crystallographic insights into the autocatalytic assembly mechanism of a bacteriophage tail spike.,Xiang Y, Leiman PG, Li L, Grimes S, Anderson DL, Rossmann MG Mol Cell. 2009 May 15;34(3):375-86. PMID:19450535^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Xiang Y, Leiman PG, Li L, Grimes S, Anderson DL, Rossmann MG. Crystallographic insights into the autocatalytic assembly mechanism of a bacteriophage tail spike. Mol Cell. 2009 May 15;34(3):375-86. PMID:19450535 doi:10.1016/j.molcel.2009.04.009

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3gqk"

Categories: Bpph2 | Rossmann, M G | Xiang, Y | Beta barrel | Viral protein

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the Bacteriophage phi29 gene product 12 C-terminal fragment in complex with ATP==
 <StructureSection load='3gqk' size='340' side='right' caption='[[3gqk]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3gq7|3gq7]], [[3gq8|3gq8]], [[3gq9|3gq9]], [[3gqa|3gqa]], [[3gqh|3gqh]], [[3gqn|3gqn]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">12, gene product 12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10756 BPPH2])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gqk OCA], [http://pdbe.org/3gqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gqk RCSB], [http://www.ebi.ac.uk/pdbsum/3gqk PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gqk OCA], [http://pdbe.org/3gqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gqk RCSB], [http://www.ebi.ac.uk/pdbsum/3gqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gqk ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==

3gqk

From Proteopedia

Revision as of 07:10, 1 November 2017

Crystal Structure of the Bacteriophage phi29 gene product 12 C-terminal fragment in complex with ATP

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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