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Publication Abstract from PubMed

In neurons, SNAREs, synaptotagmin and other factors catalyze Ca(2+)-triggered fusion of vesicles with the plasma membrane. The molecular mechanism of this process, especially the interaction between synaptotagmin and SNAREs, remains an enigma. Here we characterized this interaction by single-molecule fluorescence microscopy and crystallography. The two rigid Ca(2+)-binding domains of synaptotagmin 3 (Syt3) undergo large relative motions in solution. Interaction with SNARE complex amplifies a particular state of the two domains that is further enhanced by Ca(2+). This state is represented by the first SNARE-induced Ca(2+)-bound crystal structure of a synaptotagmin fragment containing both domains. The arrangement of the Ca(2+)-binding loops of this structure of Syt3 matches that of SNARE-bound Syt1, suggesting a conserved feature of synaptotagmins. The loops resemble the membrane-interacting loops of certain viral fusion proteins in the postfusion state, suggesting unexpected similarities between both fusion systems.

Molecular mechanism of the synaptotagmin-SNARE interaction in Ca2+-triggered vesicle fusion.,Vrljic M, Strop P, Ernst JA, Sutton RB, Chu S, Brunger AT Nat Struct Mol Biol. 2010 Mar;17(3):325-31. Epub 2010 Feb 21. PMID:20173762^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.