This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

3kf6

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 08:32, 1 November 2017

Crystal structure of S. pombe Stn1-ten1 complex

Structural highlights

3kf6 is a 2 chain structure with sequence from Cbs 356. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3key, 3kf8
Gene:	stn1, SPBC409.12c (CBS 356), ten1, SPCC1393.14 (CBS 356)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[STN1_SCHPO] Required for telomere maintenance. [TEN1_SCHPO] Required for telomere maintenance.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

In budding yeast, Cdc13, Stn1, and Ten1 form a heterotrimeric complex (CST) that is essential for telomere protection and maintenance. Previous bioinformatics analysis revealed a putative oligonucleotide/oligosaccharide-binding (OB) fold at the N terminus of Stn1 (Stn1N) that shows limited sequence similarity to the OB fold of Rpa2, a subunit of the eukaryotic ssDNA-binding protein complex replication protein A (RPA). Here we present functional and structural analyses of Stn1 and Ten1 from multiple budding and fission yeast. The crystal structure of the Candida tropicalis Stn1N complexed with Ten1 demonstrates an Rpa2N-Rpa3-like complex. In both structures, the OB folds of the two components pack against each other through interactions between two C-terminal helices. The structure of the C-terminal domain of Saccharomyces cerevisiae Stn1 (Stn1C) was found to comprise two related winged helix-turn-helix (WH) motifs, one of which is most similar to the WH motif at the C terminus of Rpa2, again supporting the notion that Stn1 resembles Rpa2. The crystal structure of the fission yeast Schizosaccharomyces pombe Stn1N-Ten1 complex exhibits a virtually identical architecture as the C. tropicalis Stn1N-Ten1. Functional analyses of the Candida albicans Stn1 and Ten1 proteins revealed critical roles for these proteins in suppressing aberrant telomerase and recombination activities at telomeres. Mutations that disrupt the Stn1-Ten1 interaction induce telomere uncapping and abolish the telomere localization of Ten1. Collectively, our structural and functional studies illustrate that, instead of being confined to budding yeast telomeres, the CST complex may represent an evolutionarily conserved RPA-like telomeric complex at the 3' overhangs that works in parallel with or instead of the well-characterized POT1-TPP1/TEBPalpha-beta complex.

Stn1-Ten1 is an Rpa2-Rpa3-like complex at telomeres.,Sun J, Yu EY, Yang Y, Confer LA, Sun SH, Wan K, Lue NF, Lei M Genes Dev. 2009 Dec 15;23(24):2900-14. PMID:20008938^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sun J, Yu EY, Yang Y, Confer LA, Sun SH, Wan K, Lue NF, Lei M. Stn1-Ten1 is an Rpa2-Rpa3-like complex at telomeres. Genes Dev. 2009 Dec 15;23(24):2900-14. PMID:20008938 doi:23/24/2900

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3kf6"

@@ Line 1: / Line 1: @@
 ==Crystal structure of S. pombe Stn1-ten1 complex==
 <StructureSection load='3kf6' size='340' side='right' caption='[[3kf6]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3key|3key]], [[3kf8|3kf8]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">stn1, SPBC409.12c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356]), ten1, SPCC1393.14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kf6 OCA], [http://pdbe.org/3kf6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kf6 RCSB], [http://www.ebi.ac.uk/pdbsum/3kf6 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kf6 OCA], [http://pdbe.org/3kf6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kf6 RCSB], [http://www.ebi.ac.uk/pdbsum/3kf6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kf6 ProSAT]</span></td></tr>
 </table>
 == Function ==

3kf6

From Proteopedia

Revision as of 08:32, 1 November 2017

Crystal structure of S. pombe Stn1-ten1 complex

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox