1zru
From Proteopedia
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= ORF 18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= ORF 18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=100641 Lactococcus lactis phage p2]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2bsd|2BSD]], [[2bse|2BSE]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zru FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zru OCA], [http://www.ebi.ac.uk/pdbsum/1zru PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zru RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZRU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1ZRU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis_phage_p2 Lactococcus lactis phage p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZRU OCA]. |
==Reference== | ==Reference== | ||
Receptor-binding protein of Lactococcus lactis phages: identification and characterization of the saccharide receptor-binding site., Tremblay DM, Tegoni M, Spinelli S, Campanacci V, Blangy S, Huyghe C, Desmyter A, Labrie S, Moineau S, Cambillau C, J Bacteriol. 2006 Apr;188(7):2400-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16547026 16547026] | Receptor-binding protein of Lactococcus lactis phages: identification and characterization of the saccharide receptor-binding site., Tremblay DM, Tegoni M, Spinelli S, Campanacci V, Blangy S, Huyghe C, Desmyter A, Labrie S, Moineau S, Cambillau C, J Bacteriol. 2006 Apr;188(7):2400-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16547026 16547026] | ||
| - | [[Category: Lactococcus lactis phage | + | [[Category: Lactococcus lactis phage p2]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Blangy, S.]] | [[Category: Blangy, S.]] | ||
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[[Category: Tegoni, M.]] | [[Category: Tegoni, M.]] | ||
[[Category: Tremblay, D M.]] | [[Category: Tremblay, D M.]] | ||
| - | [[Category: GOL]] | ||
[[Category: 3 domains: beta barrel]] | [[Category: 3 domains: beta barrel]] | ||
[[Category: beta barrel]] | [[Category: beta barrel]] | ||
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[[Category: structural proteomics in europe]] | [[Category: structural proteomics in europe]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:40:48 2008'' |
Revision as of 22:40, 30 March 2008
| |||||||
| , resolution 1.73Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | ORF 18 (Lactococcus lactis phage p2) | ||||||
| Related: | 2BSD, 2BSE
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
structure of the lactophage p2 receptor binding protein in complex with glycerol
Overview
Phage p2, a member of the lactococcal 936 phage species, infects Lactococcus lactis strains by binding initially to specific carbohydrate receptors using its receptor-binding protein (RBP). The structures of p2 RBP, a homotrimeric protein composed of three domains, and of its complex with a neutralizing llama VH domain (VHH5) have been determined (S. Spinelli, A. Desmyter, C. T. Verrips, H. J. de Haard, S. Moineau, and C. Cambillau, Nat. Struct. Mol. Biol. 13:85-89, 2006). Here, we show that VHH5 was able to neutralize 12 of 50 lactococcal phages belonging to the 936 species. Moreover, escape phage mutants no longer neutralized by VHH5 were isolated from 11 of these phages. All of the mutations (but one) cluster in the RBP/VHH5 interaction surface that delineates the receptor-binding area. A glycerol molecule, observed in the 1.7-A resolution structure of RBP, was found to bind tightly (Kd= 0.26 microM) in a crevice located in this area. Other saccharides bind RBP with comparable high affinity. These data prove the saccharidic nature of the bacterial receptor recognized by phage p2 and identify the position of its binding site in the RBP head domain.
About this Structure
1ZRU is a Single protein structure of sequence from Lactococcus lactis phage p2. Full crystallographic information is available from OCA.
Reference
Receptor-binding protein of Lactococcus lactis phages: identification and characterization of the saccharide receptor-binding site., Tremblay DM, Tegoni M, Spinelli S, Campanacci V, Blangy S, Huyghe C, Desmyter A, Labrie S, Moineau S, Cambillau C, J Bacteriol. 2006 Apr;188(7):2400-10. PMID:16547026
Page seeded by OCA on Mon Mar 31 01:40:48 2008
Categories: Lactococcus lactis phage p2 | Single protein | Blangy, S. | Cambillau, C. | Desmyter, A. | Haard, H de. | Huyghe, C. | Labrie, S. | Moineau, S. | SPINE, Structural Proteomics in Europe. | Spinelli, S. | Tegoni, M. | Tremblay, D M. | 3 domains: beta barrel | Beta barrel | Beta prism | Spine | Structural genomic | Structural proteomics in europe
