1zu1
From Proteopedia
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|PDB= 1zu1 |SIZE=350|CAPTION= <scene name='initialview01'>1zu1</scene> | |PDB= 1zu1 |SIZE=350|CAPTION= <scene name='initialview01'>1zu1</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zu1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zu1 OCA], [http://www.ebi.ac.uk/pdbsum/1zu1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zu1 RCSB]</span> | ||
}} | }} | ||
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[[Category: Moller, H M.]] | [[Category: Moller, H M.]] | ||
[[Category: Wright, P E.]] | [[Category: Wright, P E.]] | ||
| - | [[Category: ZN]] | ||
[[Category: helix-loop-helix]] | [[Category: helix-loop-helix]] | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
[[Category: zinc finger protein]] | [[Category: zinc finger protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:41:38 2008'' |
Revision as of 22:41, 30 March 2008
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of the N-terminal Zinc Fingers of the Xenopus laevis double stranded RNA binding protein ZFa
Overview
Several zinc finger proteins have been discovered recently that bind specifically to double-stranded RNA. These include the mammalian JAZ and wig proteins, and the seven-zinc finger protein ZFa from Xenopus laevis. We have determined the solution structure of a 127 residue fragment of ZFa, which consists of two zinc finger domains connected by a linker that remains unstructured in the free protein in solution. The first zinc finger consists of a three-stranded beta-sheet and three helices, while the second finger contains only a two-stranded sheet and two helices. The common structures of the core regions of the two fingers are superimposable. Each finger has a highly electropositive surface that maps to a helix-kink-helix motif. There is no evidence for interactions between the two fingers, consistent with the length (24 residues) and unstructured nature of the intervening linker. Comparison with a number of other proteins shows similarities in the topology and arrangement of secondary structure elements with canonical DNA-binding zinc fingers, with protein interaction motifs such as FOG zinc fingers, and with other DNA-binding and RNA-binding proteins that do not contain zinc. However, in none of these cases does the alignment of these structures with the ZFa zinc fingers produce a consistent picture of a plausible RNA-binding interface. We conclude that the ZFa zinc fingers represent a new motif for the binding of double-stranded RNA.
About this Structure
1ZU1 is a Single protein structure of sequence from Xenopus laevis. Full crystallographic information is available from OCA.
Reference
Solution structure of the N-terminal zinc fingers of the Xenopus laevis double-stranded RNA-binding protein ZFa., Moller HM, Martinez-Yamout MA, Dyson HJ, Wright PE, J Mol Biol. 2005 Aug 26;351(4):718-30. PMID:16051273
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