5m3z
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Citrobacter freundii methionine gamma-lyase with C115H replacement in the complex with L-norleucine== | |
| + | <StructureSection load='5m3z' size='340' side='right' caption='[[5m3z]], [[Resolution|resolution]] 1.45Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5m3z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_freundii"_braak_1928 "bacterium freundii" braak 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M3Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M3Z FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NLE:NORLEUCINE'>NLE</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=PY6:2-[O-PHOSPHONOPYRIDOXYL]-AMINO-HEXANOIC+ACID'>PY6</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AN672_11005, HMPREF3212_02785, TN42_08855, TO64_18625 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=546 "Bacterium freundii" Braak 1928])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine_gamma-lyase Methionine gamma-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.11 4.4.1.11] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m3z OCA], [http://pdbe.org/5m3z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m3z RCSB], [http://www.ebi.ac.uk/pdbsum/5m3z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m3z ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The mutant form of Citrobacter freundii methionine gamma-lyase with the replacement of active site Cys115 for His has been found to be inactive in the gamma-elimination reaction of methionine while fully active in the gamma-elimination reaction of O-acetyl-l-homoserine and in the beta-elimination reaction of S-alk(en)yl-substituted cysteines. In this work, the crystal structure of the mutant enzyme complexed with competitive inhibitor, l-norleucine was determined at 1.45A resolution. At the enzyme active site the inhibitor proved to be bound both noncovalently and covalently, which corresponds to the two intermediates of the gamma- and beta-elimination reactions, Michaelis complex and the external aldimine. Analysis of the structure allowed us to suggest the possible reason for the inability of the mutant enzyme to catalyze the physiological reaction. | ||
| - | + | Crystal structure of mutant form Cys115His of Citrobacter freundii methionine gamma-lyase complexed with l-norleucine.,Revtovich SV, Morozova EA, Kulikova VV, Anufrieva NV, Osipova TI, Koval VS, Nikulin AD, Demidkina TV Biochim Biophys Acta. 2017 Sep;1865(9):1123-1128. doi:, 10.1016/j.bbapap.2017.06.001. Epub 2017 Jun 6. PMID:28602917<ref>PMID:28602917</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5m3z" style="background-color:#fffaf0;"></div> |
| - | [[Category: Demidkina, T | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Revtovich, S | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Bacterium freundii braak 1928]] | ||
| + | [[Category: Methionine gamma-lyase]] | ||
| + | [[Category: Anufrieva, N V]] | ||
| + | [[Category: Demidkina, T V]] | ||
| + | [[Category: Morozova, E A]] | ||
| + | [[Category: Nikulin, A D]] | ||
| + | [[Category: Revtovich, S V]] | ||
| + | [[Category: C115h substitution]] | ||
| + | [[Category: L-norleucine]] | ||
| + | [[Category: Lyase]] | ||
Revision as of 11:00, 6 November 2017
Crystal structure of Citrobacter freundii methionine gamma-lyase with C115H replacement in the complex with L-norleucine
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