1zz8
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1zz8 |SIZE=350|CAPTION= <scene name='initialview01'>1zz8</scene>, resolution 2.30Å | |PDB= 1zz8 |SIZE=350|CAPTION= <scene name='initialview01'>1zz8</scene>, resolution 2.30Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene> | + | |LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=S0H:(S)-2-HYDROXYPROPYLPHOSPHONIC+ACID'>S0H</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= fom4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 Streptomyces wedmorensis]) | |GENE= fom4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 Streptomyces wedmorensis]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1zz6|1ZZ6]], [[1zz7|1ZZ7]], [[1zz9|1ZZ9]], [[1zzb|1ZZB]], [[1zzc|1ZZC]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zz8 OCA], [http://www.ebi.ac.uk/pdbsum/1zz8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zz8 RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Liu, P.]] | [[Category: Liu, P.]] | ||
[[Category: Yan, F.]] | [[Category: Yan, F.]] | ||
| - | [[Category: FE2]] | ||
| - | [[Category: S0H]] | ||
[[Category: cupin]] | [[Category: cupin]] | ||
[[Category: mononuclear iron enzyme]] | [[Category: mononuclear iron enzyme]] | ||
[[Category: substrate-enzyme complex]] | [[Category: substrate-enzyme complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:43:36 2008'' |
Revision as of 22:43, 30 March 2008
| |||||||
| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | fom4 (Streptomyces wedmorensis) | ||||||
| Related: | 1ZZ6, 1ZZ7, 1ZZ9, 1ZZB, 1ZZC
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of FeII HppE in Complex with Substrate Form 2
Overview
The biosynthetic pathway of the clinically important antibiotic fosfomycin uses enzymes that catalyse reactions without precedent in biology. Among these is hydroxypropylphosphonic acid epoxidase, which represents a new subfamily of non-haem mononuclear iron enzymes. Here we present six X-ray structures of this enzyme: the apoenzyme at 2.0 A resolution; a native Fe(II)-bound form at 2.4 A resolution; a tris(hydroxymethyl)aminomethane-Co(II)-enzyme complex structure at 1.8 A resolution; a substrate-Co(II)-enzyme complex structure at 2.5 A resolution; and two substrate-Fe(II)-enzyme complexes at 2.1 and 2.3 A resolution. These structural data lead us to suggest how this enzyme is able to recognize and respond to its substrate with a conformational change that protects the radical-based intermediates formed during catalysis. Comparisons with other family members suggest why substrate binding is able to prime iron for dioxygen binding in the absence of alpha-ketoglutarate (a co-substrate required by many mononuclear iron enzymes), and how the unique epoxidation reaction of hydroxypropylphosphonic acid epoxidase may occur.
About this Structure
1ZZ8 is a Single protein structure of sequence from Streptomyces wedmorensis. Full crystallographic information is available from OCA.
Reference
Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme., Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL, Nature. 2005 Oct 6;437(7060):838-44. Epub 2005 Jul 13. PMID:16015285
Page seeded by OCA on Mon Mar 31 01:43:36 2008
