1zzc
From Proteopedia
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|PDB= 1zzc |SIZE=350|CAPTION= <scene name='initialview01'>1zzc</scene>, resolution 1.80Å | |PDB= 1zzc |SIZE=350|CAPTION= <scene name='initialview01'>1zzc</scene>, resolution 1.80Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene> | + | |LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= fom4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 Streptomyces wedmorensis]) | |GENE= fom4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 Streptomyces wedmorensis]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1zz6|1ZZ6]], [[1zz7|1ZZ7]], [[1zz8|1ZZ8]], [[1zz9|1ZZ9]], [[1zzb|1ZZB]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zzc OCA], [http://www.ebi.ac.uk/pdbsum/1zzc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zzc RCSB]</span> | ||
}} | }} | ||
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[[Category: Liu, P.]] | [[Category: Liu, P.]] | ||
[[Category: Yan, F.]] | [[Category: Yan, F.]] | ||
| - | [[Category: CO]] | ||
| - | [[Category: TRS]] | ||
[[Category: cupin]] | [[Category: cupin]] | ||
[[Category: holo-hppe]] | [[Category: holo-hppe]] | ||
[[Category: mononuclear iron enzyme]] | [[Category: mononuclear iron enzyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:43:42 2008'' |
Revision as of 22:43, 30 March 2008
| |||||||
| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | fom4 (Streptomyces wedmorensis) | ||||||
| Related: | 1ZZ6, 1ZZ7, 1ZZ8, 1ZZ9, 1ZZB
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of CoII HppE in Complex with Tris Buffer
Overview
The biosynthetic pathway of the clinically important antibiotic fosfomycin uses enzymes that catalyse reactions without precedent in biology. Among these is hydroxypropylphosphonic acid epoxidase, which represents a new subfamily of non-haem mononuclear iron enzymes. Here we present six X-ray structures of this enzyme: the apoenzyme at 2.0 A resolution; a native Fe(II)-bound form at 2.4 A resolution; a tris(hydroxymethyl)aminomethane-Co(II)-enzyme complex structure at 1.8 A resolution; a substrate-Co(II)-enzyme complex structure at 2.5 A resolution; and two substrate-Fe(II)-enzyme complexes at 2.1 and 2.3 A resolution. These structural data lead us to suggest how this enzyme is able to recognize and respond to its substrate with a conformational change that protects the radical-based intermediates formed during catalysis. Comparisons with other family members suggest why substrate binding is able to prime iron for dioxygen binding in the absence of alpha-ketoglutarate (a co-substrate required by many mononuclear iron enzymes), and how the unique epoxidation reaction of hydroxypropylphosphonic acid epoxidase may occur.
About this Structure
1ZZC is a Single protein structure of sequence from Streptomyces wedmorensis. Full crystallographic information is available from OCA.
Reference
Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme., Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL, Nature. 2005 Oct 6;437(7060):838-44. Epub 2005 Jul 13. PMID:16015285
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