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5v8w

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Revision as of 14:40, 6 November 2017

Crystal structure of human Integrator IntS9-IntS11 CTD complex

Structural highlights

5v8w is a 8 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	INTS9, RC74 (HUMAN), CPSF3L, INTS11, RC68 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[INT9_HUMAN] Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. [INT11_HUMAN] Catalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage.^[1]

Publication Abstract from PubMed

The metazoan Integrator complex (INT) has important functions in the 3'-end processing of noncoding RNAs, including the uridine-rich small nuclear RNA (UsnRNA) and enhancer RNA (eRNA), and in the transcription of coding genes by RNA polymerase II. The INT contains at least 14 subunits, but its molecular mechanism of action is poorly understood, because currently there is little structural information about its subunits. The endonuclease activity of INT is mediated by its subunit 11 (IntS11), which belongs to the metallo-beta-lactamase superfamily and is a paralog of CPSF-73, the endonuclease for pre-mRNA 3'-end processing. IntS11 forms a stable complex with Integrator complex subunit 9 (IntS9) through their C-terminal domains (CTDs). Here, we report the crystal structure of the IntS9-IntS11 CTD complex at 2.1-A resolution and detailed, structure-based biochemical and functional studies. The complex is composed of a continuous nine-stranded beta-sheet with four strands from IntS9 and five from IntS11. Highly conserved residues are located in the extensive interface between the two CTDs. Yeast two-hybrid assays and coimmunoprecipitation experiments confirm the structural observations on the complex. Functional studies demonstrate that the IntS9-IntS11 interaction is crucial for the role of INT in snRNA 3'-end processing.

Molecular basis for the interaction between Integrator subunits IntS9 and IntS11 and its functional importance.,Wu Y, Albrecht TR, Baillat D, Wagner EJ, Tong L Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):4394-4399. doi:, 10.1073/pnas.1616605114. Epub 2017 Apr 10. PMID:28396433^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Baillat D, Hakimi MA, Naar AM, Shilatifard A, Cooch N, Shiekhattar R. Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II. Cell. 2005 Oct 21;123(2):265-76. PMID:16239144 doi:http://dx.doi.org/S0092-8674(05)00824-X
↑ Wu Y, Albrecht TR, Baillat D, Wagner EJ, Tong L. Molecular basis for the interaction between Integrator subunits IntS9 and IntS11 and its functional importance. Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):4394-4399. doi:, 10.1073/pnas.1616605114. Epub 2017 Apr 10. PMID:28396433 doi:http://dx.doi.org/10.1073/pnas.1616605114

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5v8w"

@@ Line 3: / Line 3: @@
 <StructureSection load='5v8w' size='340' side='right' caption='[[5v8w]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5v8w]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V8W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5V8W FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5v8w]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V8W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5V8W FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5v8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v8w OCA], [http://pdbe.org/5v8w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v8w RCSB], [http://www.ebi.ac.uk/pdbsum/5v8w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v8w ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">INTS9, RC74 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CPSF3L, INTS11, RC68 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5v8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v8w OCA], [http://pdbe.org/5v8w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v8w RCSB], [http://www.ebi.ac.uk/pdbsum/5v8w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v8w ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
 [[Category: Tong, L]]
 [[Category: Wu, Y]]

5v8w

From Proteopedia

Revision as of 14:40, 6 November 2017

Crystal structure of human Integrator IntS9-IntS11 CTD complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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