2a1k
From Proteopedia
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|PDB= 2a1k |SIZE=350|CAPTION= <scene name='initialview01'>2a1k</scene>, resolution 2.000Å | |PDB= 2a1k |SIZE=350|CAPTION= <scene name='initialview01'>2a1k</scene>, resolution 2.000Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= 32 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= 32 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12353 Enterobacteria phage RB69]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a1k OCA], [http://www.ebi.ac.uk/pdbsum/2a1k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a1k RCSB]</span> | ||
}} | }} | ||
| Line 16: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2A1K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 2A1K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb69 Enterobacteria phage rb69]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A1K OCA]. |
==Reference== | ==Reference== | ||
Structure and enzymatic properties of a chimeric bacteriophage RB69 DNA polymerase and single-stranded DNA binding protein with increased processivity., Sun S, Geng L, Shamoo Y, Proteins. 2006 Oct 1;65(1):231-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16881051 16881051] | Structure and enzymatic properties of a chimeric bacteriophage RB69 DNA polymerase and single-stranded DNA binding protein with increased processivity., Sun S, Geng L, Shamoo Y, Proteins. 2006 Oct 1;65(1):231-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16881051 16881051] | ||
| - | [[Category: Enterobacteria phage | + | [[Category: Enterobacteria phage rb69]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Geng, L.]] | [[Category: Geng, L.]] | ||
[[Category: Shamoo, Y.]] | [[Category: Shamoo, Y.]] | ||
[[Category: Sun, S.]] | [[Category: Sun, S.]] | ||
| - | [[Category: ZN]] | ||
[[Category: 5-stranded beta-sheet]] | [[Category: 5-stranded beta-sheet]] | ||
[[Category: ob fold]] | [[Category: ob fold]] | ||
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[[Category: zn2+ binding subdomain]] | [[Category: zn2+ binding subdomain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:46:34 2008'' |
Revision as of 22:46, 30 March 2008
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| , resolution 2.000Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | 32 (Enterobacteria phage RB69) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RB69 single-stranded DNA binding protein core domain
Overview
In vivo, replicative DNA polymerases are made more processive by their interactions with accessory proteins at the replication fork. Single-stranded DNA binding protein (SSB) is an essential protein that binds tightly and cooperatively to single-stranded DNA during replication to remove adventitious secondary structures and protect the exposed DNA from endogenous nucleases. Using information from high resolution structures and biochemical data, we have engineered a functional chimeric enzyme of the bacteriophage RB69 DNA polymerase and SSB with substantially increased processivity. Fusion of RB69 DNA polymerase with its cognate SSB via a short six amino acid linker increases affinity for primer-template DNA by sixfold and subsequently increases processivity by sevenfold while maintaining fidelity. The crystal structure of this fusion protein was solved by a combination of multiwavelength anomalous diffraction and molecular replacement to 3.2 A resolution and shows that RB69 SSB is positioned proximal to the N-terminal domain of RB69 DNA polymerase near the template strand channel. The structural and biochemical data suggest that SSB interactions with DNA polymerase are transient and flexible, consistent with models of a dynamic replisome during elongation.
About this Structure
2A1K is a Single protein structure of sequence from Enterobacteria phage rb69. Full crystallographic information is available from OCA.
Reference
Structure and enzymatic properties of a chimeric bacteriophage RB69 DNA polymerase and single-stranded DNA binding protein with increased processivity., Sun S, Geng L, Shamoo Y, Proteins. 2006 Oct 1;65(1):231-8. PMID:16881051
Page seeded by OCA on Mon Mar 31 01:46:34 2008
