2a57
From Proteopedia
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|PDB= 2a57 |SIZE=350|CAPTION= <scene name='initialview01'>2a57</scene>, resolution 2.750Å | |PDB= 2a57 |SIZE=350|CAPTION= <scene name='initialview01'>2a57</scene>, resolution 2.750Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=CRM:3-[8-((2S,3S,4R)-2,3,4,5-TETRAHYDROXYPENTYL)-2,4,7-TRIOXO-1,3,8-TRIHYDROPTERIDIN-6-YL]PROPANOIC+ACID'>CRM</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] </span> |
|GENE= rib4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]) | |GENE= rib4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1kyv|1KYV]], [[1kyx|1KYX]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a57 OCA], [http://www.ebi.ac.uk/pdbsum/2a57 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a57 RCSB]</span> | ||
}} | }} | ||
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[[Category: Koch, M.]] | [[Category: Koch, M.]] | ||
[[Category: Weber, S.]] | [[Category: Weber, S.]] | ||
| - | + | [[Category: 6,7-dimethyl-8-ribityllumazine synthase]] | |
| - | + | ||
| - | [[Category: 6 | + | |
| - | + | ||
[[Category: lusy]] | [[Category: lusy]] | ||
[[Category: riboflavin]] | [[Category: riboflavin]] | ||
[[Category: riboflavin biosynthesis]] | [[Category: riboflavin biosynthesis]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:47:59 2008'' |
Revision as of 22:48, 30 March 2008
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| , resolution 2.750Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | rib4 (Schizosaccharomyces pombe) | ||||||
| Activity: | Riboflavin synthase, with EC number 2.5.1.9 | ||||||
| Related: | 1KYV, 1KYX
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of 6,7-Dimthyl-8-ribityllumazine synthase from Schizosaccharomyces pombe mutant W27Y with bound ligand 6-carboxyethyl-7-oxo-8-ribityllumazine
Overview
The amino acid residue tryptophan 27 of 6,7-dimethyl-8-ribityllumazine synthase of the yeast Schizosaccharomyces pombe was replaced by tyrosine. The structures of the W27Y mutant protein in complex with riboflavin, the substrate analogue 5-nitroso-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, and the product analogue 6-carboxyethyl-7-oxo-8-ribityllumazine, were determined by X-ray crystallography at resolutions of 2.7-2.8 A. Whereas the indole system of W27 forms a coplanar pi-complex with riboflavin, the corresponding phenyl ring in the W27Y mutant establishes only peripheral contact with the heterocyclic ring system of the bound riboflavin. These findings provide an explanation for the absence of the long wavelength shift in optical absorption spectra of riboflavin bound to the mutant enzyme. The structures of the mutants are important tools for the interpretation of the unusual physical properties of riboflavin in complex with lumazine synthase.
About this Structure
2A57 is a Single protein structure of sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.
Reference
Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase., Koch M, Breithaupt C, GerhardtHaase S, Weber S, Cushman M, Huber R, Bacher A, Fischer M, Eur J Biochem. 2004 Aug;271(15):3208-14. PMID:15265040
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