2a5m
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= Crygs ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | |GENE= Crygs ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1zwm|1ZWM]], [[1zwo|1ZWO]], [[1amm|1AMM]], [[1hk0|1HK0]], [[1a7h|1A7H]], [[1a45|1A45]], [[1ag4|1AG4]], [[1a5d|1A5D]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a5m OCA], [http://www.ebi.ac.uk/pdbsum/2a5m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a5m RCSB]</span> | ||
}} | }} | ||
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[[Category: small-angle x-ray scattering]] | [[Category: small-angle x-ray scattering]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:48:11 2008'' |
Revision as of 22:48, 30 March 2008
| |||||||
| Gene: | Crygs (Mus musculus) | ||||||
| Related: | 1ZWM, 1ZWO, 1AMM, 1HK0, 1A7H, 1A45, 1AG4, 1A5D
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR structure of murine gamma-S crystallin from joint refinement with SAXS data
Overview
Determination of the 3D structures of multidomain proteins by solution NMR methods presents a number of unique challenges related to their larger molecular size and the usual scarcity of constraints at the interdomain interface, often resulting in a decrease in structural accuracy. In this respect, experimental information from small-angle scattering of X-ray radiation in solution (SAXS) presents a suitable complement to the NMR data, as it provides an independent constraint on the overall molecular shape. A computational procedure is described that allows incorporation of such SAXS data into the mainstream high-resolution macromolecular structure refinement. The method is illustrated for a two-domain 177-amino-acid protein, gammaS crystallin, using an experimental SAXS data set fitted at resolutions from approximately 200 A to approximately 30 A. Inclusion of these data during structure refinement decreases the backbone coordinate root-mean-square difference between the derived model and the high-resolution crystal structure of a 54% homologous gammaB crystallin from 1.96 +/- 0.07 A to 1.31 +/- 0.04 A. Combining SAXS data with NMR restraints can be accomplished at a moderate computational expense and is expected to become useful for multidomain proteins, multimeric assemblies, and tight macromolecular complexes.
About this Structure
2A5M is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Refinement of multidomain protein structures by combination of solution small-angle X-ray scattering and NMR data., Grishaev A, Wu J, Trewhella J, Bax A, J Am Chem Soc. 2005 Nov 30;127(47):16621-8. PMID:16305251
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