2a5r
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2a5r |SIZE=350|CAPTION= <scene name='initialview01'>2a5r</scene> | |PDB= 2a5r |SIZE=350|CAPTION= <scene name='initialview01'>2a5r</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=POH:5,10,15,20-TETRA-(4-N-METHYLPYRIDYL) PORPHINE'>POH</scene> | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DI:2'-DEOXYINOSINE-5'-MONOPHOSPHATE'>DI</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=POH:5,10,15,20-TETRA-(4-N-METHYLPYRIDYL)+PORPHINE'>POH</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1eeg|1EEG]], [[1jjp|1JJP]], [[1y8d|1Y8D]], [[1d6d|1D6D]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a5r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a5r OCA], [http://www.ebi.ac.uk/pdbsum/2a5r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a5r RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Patel, D J.]] | [[Category: Patel, D J.]] | ||
[[Category: Phan, A T.]] | [[Category: Phan, A T.]] | ||
| - | [[Category: | + | [[Category: c-myc promoter 3+1 g-tetrad]] |
| - | [[Category: monomeric parallel-stranded quadruplex | + | [[Category: gaag loop]] |
| + | [[Category: gag triad]] | ||
| + | [[Category: monomeric parallel-stranded quadruplex]] | ||
| + | [[Category: single nucleotide chain reversal loop]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:48:14 2008'' |
Revision as of 22:48, 30 March 2008
| |||||||
| Ligands: | , , , , | ||||||
| Related: | 1EEG, 1JJP, 1Y8D, 1D6D
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Complex of tetra-(4-n-methylpyridyl) porphin with monomeric parallel-stranded DNA tetraplex, snap-back 3+1 3' G-tetrad, single-residue chain reversal loops, GAG triad in the context of GAAG diagonal loop, C-MYC promoter, NMR, 6 struct.
Overview
It has been widely accepted that DNA can adopt other biologically relevant structures beside the Watson-Crick double helix. One recent important example is the guanine-quadruplex (G-quadruplex) structure formed by guanine tracts found in the MYC (or c-myc) promoter region, which regulates the transcription of the MYC oncogene. Stabilization of this G-quadruplex by ligands, such as the cationic porphyrin TMPyP4, decreases the transcriptional level of MYC. Here, we report the first structure of a DNA fragment containing five guanine tracts from this region. An unusual G-quadruplex fold, which was derived from NMR restraints using unambiguous model-independent resonance assignment approaches, involves a core of three stacked guanine tetrads formed by four parallel guanine tracts with all anti guanines and a snapback 3'-end syn guanine. We have determined the structure of the complex formed between this G-quadruplex and TMPyP4. This structural information, combined with details of small-molecule interaction, provides a platform for the design of anticancer drugs targeting multi-guanine-tract sequences that are found in the MYC and other oncogenic promoters, as well as in telomeres.
About this Structure
2A5R is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Small-molecule interaction with a five-guanine-tract G-quadruplex structure from the human MYC promoter., Phan AT, Kuryavyi V, Gaw HY, Patel DJ, Nat Chem Biol. 2005 Aug;1(3):167-73. Epub 2005 Jul 17. PMID:16408022
Page seeded by OCA on Mon Mar 31 01:48:14 2008
