3nsg

Publication Abstract from PubMed

OmpF is a major general diffusion porin of Salmonella typhi, a Gram-negative bacterium, which is an obligatory human pathogen causing typhoid. The structure of S. typhi Ty21a OmpF (PDB Id: 3NSG) determined at 2.8A resolution by X-ray crystallography shows a 16-stranded beta-barrel with three beta-barrel monomers associated to form a trimer. The packing observed in S. typhi Ty21a rfOmpF crystals has not been observed earlier in other porin structures. The variations seen in the loop regions provide a starting point for using the S. typhi OmpF for structure-based multi-valent vaccine design. Along one side of the S. typhi Ty21a OmpF pore there exists a staircase arrangement of basic residues (20R, 60R, 62K, 65R, 77R, 130R and 16K), which also contribute, to the electrostatic potential in the pore. This structure suggests the presence of asymmetric electrostatics in the porin oligomer. Moreover, antibiotic translocation, permeability and reduced uptake in the case of mutants can be understood based on the structure paving the way for designing new antibiotics.

Asymmetric pore occupancy in crystal structure of OmpF porin from Salmonella typhi.,Balasubramaniam D, Arockiasamy A, Kumar PD, Sharma A, Krishnaswamy S J Struct Biol. 2012 Jun;178(3):233-44. Epub 2012 Apr 16. PMID:22525817^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.