5lem
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of DARPin-DARPin rigid fusion, variant DD_Off7_11_3G124 in complex with Maltose-binding Protein and Green Fluorescent Protein== | |
| - | + | <StructureSection load='5lem' size='340' side='right' caption='[[5lem]], [[Resolution|resolution]] 2.98Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[5lem]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi], [http://en.wikipedia.org/wiki/Eco57 Eco57] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LEM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LEM FirstGlance]. <br> | |
| - | + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr> | |
| - | [[Category: | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">malE, Z5632, ECs5017 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57]), GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lem OCA], [http://pdbe.org/5lem PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lem RCSB], [http://www.ebi.ac.uk/pdbsum/5lem PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lem ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. [[http://www.uniprot.org/uniprot/MALE_ECO57 MALE_ECO57]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides (By similarity). | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Aeqvi]] | ||
| + | [[Category: Eco57]] | ||
| + | [[Category: Synthetic construct sequences]] | ||
| + | [[Category: Batyuk, A]] | ||
| + | [[Category: Mittl, P R]] | ||
| + | [[Category: Plueckthun, A]] | ||
| + | [[Category: Wu, Y]] | ||
| + | [[Category: Designed ankyrin repeat protein]] | ||
| + | [[Category: Fluorescent protein]] | ||
| + | [[Category: Protein design]] | ||
| + | [[Category: Protein engineering]] | ||
| + | [[Category: Rigid domain fusion]] | ||
| + | [[Category: X-ray crystallography]] | ||
Revision as of 08:00, 13 November 2017
Crystal structure of DARPin-DARPin rigid fusion, variant DD_Off7_11_3G124 in complex with Maltose-binding Protein and Green Fluorescent Protein
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