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Publication Abstract from PubMed

Adipose phospholipase A(2) (AdPLA or Group XVI PLA(2)) plays an important role in the onset of obesity by suppressing adipose tissue lipolysis. As a consequence, AdPLA-deficient mice are resistant to obesity induced by high-fat diet or by leptin deficiency. It has been proposed that AdPLA mediates its anti-lipolytic effects by catalyzing the release of arachidonic acid. Based on sequence homology, AdPLA is part of a small family of acyltransferases and phospholipases related to lecithin:retinol acyltransferases (LRAT). To better understand the enzymatic mechanism of AdPLA and related LRAT proteins, we solved the crystal structure of AdPLA. Our model indicates that AdPLA is structurally related to the NlpC/P60 family of cysteine proteases, having its secondary structure elements configured in a circular permutation of the classic papain fold. Using both structural and biochemical evidence we demonstrate that the enzymatic activity of AdPLA is mediated by a distinctive Cys-His-His catalytic triad and that the C-terminal transmembrane domain of AdPLA is required for the interfacial catalysis. Analysis of the enzymatic activity of AdPLA toward synthetic and natural substrates indicates that AdPLA displays PLA(1) in addition to PLA(2) activity. Thus our results provide insight into the enzymatic mechanism and biochemical properties of AdPLA and LRAT-related proteins, and lead us to propose an alternate mechanism for AdPLA in promoting adipose tissue lipolysis that is not contingent on the release of arachidonic acid and which is compatible with its combined PLA(1)/A(2) activity.

Structure/Function Relationships of Adipose Phospholipase A2 Containing a Cys-His-His Catalytic Triad.,Pang XY, Cao J, Addington L, Lovell S, Battaile KP, Zhang N, Rao JL, Dennis EA, Moise AR J Biol Chem. 2012 Aug 25. PMID:22923616^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.