2ahj
From Proteopedia
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|PDB= 2ahj |SIZE=350|CAPTION= <scene name='initialview01'>2ahj</scene>, resolution 1.7Å | |PDB= 2ahj |SIZE=350|CAPTION= <scene name='initialview01'>2ahj</scene>, resolution 1.7Å | ||
|SITE= <scene name='pdbsite=1:In+Chain+C'>1</scene>, <scene name='pdbsite=CTA:Non-Heme+Fe+Center+And+Catalytic+Site+Site_description+I+...'>CTA</scene> and <scene name='pdbsite=CTB:In+Chain+A+Site_identifier+Ctb+Site_description+Non-Heme+...'>CTB</scene> | |SITE= <scene name='pdbsite=1:In+Chain+C'>1</scene>, <scene name='pdbsite=CTA:Non-Heme+Fe+Center+And+Catalytic+Site+Site_description+I+...'>CTA</scene> and <scene name='pdbsite=CTB:In+Chain+A+Site_identifier+Ctb+Site_description+Non-Heme+...'>CTB</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=NO:NITROGEN+OXIDE'>NO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrile_hydratase Nitrile hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.84 4.2.1.84] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ahj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahj OCA], [http://www.ebi.ac.uk/pdbsum/2ahj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ahj RCSB]</span> | ||
}} | }} | ||
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[[Category: Tsujimura, M.]] | [[Category: Tsujimura, M.]] | ||
[[Category: Yohda, M.]] | [[Category: Yohda, M.]] | ||
| - | [[Category: DIO]] | ||
| - | [[Category: FE]] | ||
| - | [[Category: NO]] | ||
| - | [[Category: SO4]] | ||
| - | [[Category: ZN]] | ||
[[Category: hydratase]] | [[Category: hydratase]] | ||
[[Category: lyase]] | [[Category: lyase]] | ||
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[[Category: post-translational modification of cysteine residue]] | [[Category: post-translational modification of cysteine residue]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:52:44 2008'' |
Revision as of 22:52, 30 March 2008
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| , resolution 1.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , and | ||||||
| Ligands: | , , , , , , | ||||||
| Activity: | Nitrile hydratase, with EC number 4.2.1.84 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE
Overview
The iron-containing nitrile hydratase (NHase) is a photoreactive enzyme that is inactivated in the dark because of persistent association with NO and activated by photo-dissociation of NO. The crystal structure at 1.7 A resolution and mass spectrometry revealed the structure of the non-heme iron catalytic center in the nitrosylated state. Two Cys residues coordinated to the iron were post-translationally modified to Cys-sulfenic and -sulfinic acids. Together with another oxygen atom of the Ser ligand, these modifications induced a claw setting of oxygen atoms capturing an NO molecule. This unprecedented structure is likely to enable the photo-regulation of NHase and will provide an excellent model for designing photo-controllable chelate complexes and, ultimately, proteins.
About this Structure
2AHJ is a Protein complex structure of sequences from Rhodococcus erythropolis. Full crystallographic information is available from OCA.
Reference
Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms., Nagashima S, Nakasako M, Dohmae N, Tsujimura M, Takio K, Odaka M, Yohda M, Kamiya N, Endo I, Nat Struct Biol. 1998 May;5(5):347-51. PMID:9586994
Page seeded by OCA on Mon Mar 31 01:52:44 2008
Categories: Nitrile hydratase | Protein complex | Rhodococcus erythropolis | Dohmae, N. | Endo, I. | Kamiya, N. | Nagashima, S. | Nakasako, M. | Odaka, M. | Takio, K. | Tsujimura, M. | Yohda, M. | Hydratase | Lyase | Nitric oxide binding enzyme | Non-heme iron center | Photoreactive enzyme | Post-translational modification of cysteine residue
