2ahq
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= rpoN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | |GENE= rpoN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ahq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahq OCA], [http://www.ebi.ac.uk/pdbsum/2ahq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ahq RCSB]</span> | ||
}} | }} | ||
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[[Category: Pelton, J G.]] | [[Category: Pelton, J G.]] | ||
[[Category: Wemmer, D E.]] | [[Category: Wemmer, D E.]] | ||
| - | [[Category: rna polymerase | + | [[Category: rna polymerase,]] |
| - | + | [[Category: sigma-54,sigma factor]] | |
| - | [[Category: sigma-54]] | + | |
[[Category: solution structure]] | [[Category: solution structure]] | ||
[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:52:50 2008'' |
Revision as of 22:52, 30 March 2008
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| Gene: | rpoN (Aquifex aeolicus) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of the C-terminal RpoN Domain of Sigma-54 from Aquifex aeolicus
Overview
The "sigma" subunit of prokaryotic RNA polymerase allows gene-specific transcription initiation. Two sigma families have been identified, sigma70 and sigma54, which use distinct mechanisms to initiate transcription and share no detectable sequence homology. Although the sigma70-type factors have been well characterized structurally by x-ray crystallography, no high resolution structural information is available for the sigma54-type factors. Here we present the NMR-derived structure of the C-terminal domain of sigma54 from Aquifex aeolicus. This domain (Thr-323 to Gly-389), which contains the highly conserved RpoN box sequence, consists of a poorly structured N-terminal tail followed by a three-helix bundle, which is surprisingly similar to domains of the sigma70-type proteins. Residues of the RpoN box, which have previously been shown to be critical for DNA binding, form the second helix of an unpredicted helix-turn-helix motif. The homology of this structure with other DNA-binding proteins, combined with previous biochemical data, suggests how the C-terminal domain of sigma54 binds to DNA.
About this Structure
2AHQ is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
The C-terminal RpoN domain of sigma54 forms an unpredicted helix-turn-helix motif similar to domains of sigma70., Doucleff M, Malak LT, Pelton JG, Wemmer DE, J Biol Chem. 2005 Dec 16;280(50):41530-6. Epub 2005 Oct 6. PMID:16210314
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