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2aib
From Proteopedia
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|PDB= 2aib |SIZE=350|CAPTION= <scene name='initialview01'>2aib</scene>, resolution 1.10Å | |PDB= 2aib |SIZE=350|CAPTION= <scene name='initialview01'>2aib</scene>, resolution 1.10Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ERG:ERGOSTEROL'>ERG</scene>, <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ERG:ERGOSTEROL'>ERG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= CIN1B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4785 Phytophthora cinnamomi]) | |GENE= CIN1B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4785 Phytophthora cinnamomi]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2a8f|2A8F]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aib OCA], [http://www.ebi.ac.uk/pdbsum/2aib PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2aib RCSB]</span> | ||
}} | }} | ||
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[[Category: Sousa, N.]] | [[Category: Sousa, N.]] | ||
[[Category: Thomaz, M.]] | [[Category: Thomaz, M.]] | ||
| - | [[Category: ERG]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: MES]] | ||
[[Category: elicitin]] | [[Category: elicitin]] | ||
[[Category: phytopathogen]] | [[Category: phytopathogen]] | ||
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[[Category: sterol carrier protein]] | [[Category: sterol carrier protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:53:07 2008'' |
Revision as of 22:53, 30 March 2008
| |||||||
| , resolution 1.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | CIN1B (Phytophthora cinnamomi) | ||||||
| Related: | 2A8F
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
beta-cinnamomin in complex with ergosterol
Overview
The crystal structure of the elicitin beta-cinnamomin (beta-CIN) was determined in complex with ergosterol at 1.1 A resolution. beta-CIN/ergosterol complex crystallized in the monoclinic space group P2(1), with unit cell parameters of a = 31.0, b = 62.8, c = 50.0 A and beta = 93.4 degrees and two molecules in the asymmetric unit. Ligand extraction with chloroform followed by crystallographic analysis yielded a 1.35 A structure of beta-CIN (P4(3)2(1)2 space group) where the characteristic elicitin fold was kept. After incubation with cholesterol, a new complex structure was obtained, showing that the protein retains, after the extraction procedure, its ability to complex sterols. The necrotic effect of beta-CIN on tobacco was also shown to remain unchanged. Theoretical docking studies of the triterpene lupeol to beta-CIN provided an explanation for the apparent inability of beta-CIN to bind this ligand, as observed experimentally.
About this Structure
2AIB is a Single protein structure of sequence from Phytophthora cinnamomi. Full crystallographic information is available from OCA.
Reference
Crystal structures of the free and sterol-bound forms of beta-cinnamomin., Rodrigues ML, Archer M, Martel P, Miranda S, Thomaz M, Enguita FJ, Baptista RP, Pinho e Melo E, Sousa N, Cravador A, Carrondo MA, Biochim Biophys Acta. 2006 Jan;1764(1):110-21. Epub 2005 Oct 6. PMID:16249127
Page seeded by OCA on Mon Mar 31 01:53:07 2008
