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1am2

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Revision as of 10:46, 15 November 2017

GYRA INTEIN FROM MYCOBACTERIUM XENOPI

Structural highlights

1am2 is a 1 chain structure with sequence from "mycobacterium_xenopei"_(sic)_schwabacher_1959 "mycobacterium xenopei" (sic) schwabacher 1959. The November 2010 RCSB PDB Molecule of the Month feature on Inteins by David Goodsell is 10.2210/rcsb_pdb/mom_2010_11. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	RTP ("Mycobacterium xenopei" (sic) Schwabacher 1959)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GYRA_MYCXE] DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Several genes from prokaryotes and lower eukaryotes have been found to contain an in-frame open reading frame, which encodes for an internal protein (intein). Post-translationally, the internal polypeptide auto-splices and ligates the external sequences to yield a functional external protein (extein) and an intein. Most, but not all inteins, contain, apart from a splicing domain, a separate endonucleolytic domain that enables them to maintain their presence by a homing mechanism. We report here the crystal structure of an intein found in the gyrase A subunit from Mycobacterium xenopi at 2.2 A resolution. The structure contains an unusual beta-fold with the catalytic splice junctions at the ends of two adjacent antiparallel beta-strands. The arrangement of the active site residues Ser 1, Thr 72, His 75, His 197, and Asn 198 is consistent with a four-step mechanism for the cleavage-ligation reaction. Using site-directed mutagenesis, the N-terminal cysteine, proposed as the nucleophile in the first step of the splicing reaction, was changed to a Ser 1 and Ala 0, thus capturing the intein in a pre-spliced state.

Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing.,Klabunde T, Sharma S, Telenti A, Jacobs WR Jr, Sacchettini JC Nat Struct Biol. 1998 Jan;5(1):31-6. PMID:9437427^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Klabunde T, Sharma S, Telenti A, Jacobs WR Jr, Sacchettini JC. Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing. Nat Struct Biol. 1998 Jan;5(1):31-6. PMID:9437427

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1am2"

@@ Line 1: / Line 1: @@
 ==GYRA INTEIN FROM MYCOBACTERIUM XENOPI==
 <StructureSection load='1am2' size='340' side='right' caption='[[1am2]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[1am2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"mycobacterium_xenopei"_(sic)_schwabacher_1959 "mycobacterium xenopei" (sic) schwabacher 1959]. The November 2010 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Inteins''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2010_11 10.2210/rcsb_pdb/mom_2010_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AM2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AM2 FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RTP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1789 "Mycobacterium xenopei" (sic) Schwabacher 1959])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1am2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1am2 OCA], [http://pdbe.org/1am2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1am2 RCSB], [http://www.ebi.ac.uk/pdbsum/1am2 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1am2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1am2 OCA], [http://pdbe.org/1am2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1am2 RCSB], [http://www.ebi.ac.uk/pdbsum/1am2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1am2 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 37: / Line 38: @@
 [[Category: Sharma, S]]
 [[Category: Intein]]
+[[Category: Protein splicing]]

1am2

From Proteopedia

Revision as of 10:46, 15 November 2017

GYRA INTEIN FROM MYCOBACTERIUM XENOPI

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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