2an6
From Proteopedia
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|PDB= 2an6 |SIZE=350|CAPTION= <scene name='initialview01'>2an6</scene>, resolution 3.00Å | |PDB= 2an6 |SIZE=350|CAPTION= <scene name='initialview01'>2an6</scene>, resolution 3.00Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2an6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2an6 OCA], [http://www.ebi.ac.uk/pdbsum/2an6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2an6 RCSB]</span> | ||
}} | }} | ||
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[[Category: Parker, M W.]] | [[Category: Parker, M W.]] | ||
[[Category: Polekhina, G.]] | [[Category: Polekhina, G.]] | ||
| - | [[Category: ZN]] | ||
[[Category: protein-peptide complex]] | [[Category: protein-peptide complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:54:47 2008'' |
Revision as of 22:54, 30 March 2008
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| , resolution 3.00Å | |||||||
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Protein-peptide complex
Overview
The Siah family of RING proteins function as ubiquitin ligase components, contributing to the degradation of multiple targets involved in cell growth, differentiation, angiogenesis, oncogenesis, and inflammation. Previously, a binding motif (degron) was recognized in many of the Siah degradation targets, suggesting that Siah itself may facilitate substrate recognition. We report the crystal structure of the Siah in complex with a peptide containing the degron motif. Binding is within a groove formed in part by the zinc fingers and the first two beta strands of the TRAF-C domain of Siah. We show that residues in the degron, previously described to facilitate binding to Siah, interact with the protein. Mutagenesis of Siah at sites of interaction also abrogates both in vitro peptide binding and destabilization of a known Siah target.
About this Structure
2AN6 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Elucidation of the substrate binding site of Siah ubiquitin ligase., House CM, Hancock NC, Moller A, Cromer BA, Fedorov V, Bowtell DD, Parker MW, Polekhina G, Structure. 2006 Apr;14(4):695-701. PMID:16615911
Page seeded by OCA on Mon Mar 31 01:54:47 2008
