This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2aqj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 4: Line 4:
|PDB= 2aqj |SIZE=350|CAPTION= <scene name='initialview01'>2aqj</scene>, resolution 1.800&Aring;
|PDB= 2aqj |SIZE=350|CAPTION= <scene name='initialview01'>2aqj</scene>, resolution 1.800&Aring;
|SITE=
|SITE=
-
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene> and <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene>
+
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene>
|ACTIVITY=
|ACTIVITY=
|GENE= prnA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens])
|GENE= prnA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens])
 +
|DOMAIN=
 +
|RELATEDENTRY=[[2apg|2APG]]
 +
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aqj OCA], [http://www.ebi.ac.uk/pdbsum/2aqj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2aqj RCSB]</span>
}}
}}
Line 29: Line 32:
[[Category: SSPF, Scottish Structural Proteomics Facility.]]
[[Category: SSPF, Scottish Structural Proteomics Facility.]]
[[Category: Unversucht, S.]]
[[Category: Unversucht, S.]]
-
[[Category: CL]]
 
-
[[Category: FAD]]
 
-
[[Category: TRP]]
 
[[Category: flavin-dependent halogenase]]
[[Category: flavin-dependent halogenase]]
[[Category: helical bundle]]
[[Category: helical bundle]]
Line 40: Line 40:
[[Category: tryptophan 7-halogenase]]
[[Category: tryptophan 7-halogenase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:52:37 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:55:56 2008''

Revision as of 22:55, 30 March 2008

Image:2aqj.gif


PDB ID 2aqj

Drag the structure with the mouse to rotate
, resolution 1.800Å
Ligands: , ,
Gene: prnA (Pseudomonas fluorescens)
Related: 2APG


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



The structure of tryptophan 7-halogenase (PrnA) suggests a mechanism for regioselective chlorination


Overview

Chlorinated natural products include vancomycin and cryptophycin A. Their biosynthesis involves regioselective chlorination by flavin-dependent halogenases. We report the structural characterization of tryptophan 7-halogenase (PrnA), which regioselectively chlorinates tryptophan. Tryptophan and flavin adenine dinucleotide (FAD) are separated by a 10 angstrom-long tunnel and bound by distinct enzyme modules. The FAD module is conserved in halogenases and is related to flavin-dependent monooxygenases. On the basis of biochemical studies, crystal structures, and by analogy with monooxygenases, we predict that FADH2 reacts with O2 to make peroxyflavin, which is decomposed by Cl-. The resulting HOCl is guided through the tunnel to tryptophan, where it is activated to participate in electrophilic aromatic substitution.

About this Structure

2AQJ is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.

Reference

Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination., Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH, Science. 2005 Sep 30;309(5744):2216-9. PMID:16195462

Page seeded by OCA on Mon Mar 31 01:55:56 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2aqj"
Personal tools