2ar8
From Proteopedia
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|PDB= 2ar8 |SIZE=350|CAPTION= <scene name='initialview01'>2ar8</scene>, resolution 2.200Å | |PDB= 2ar8 |SIZE=350|CAPTION= <scene name='initialview01'>2ar8</scene>, resolution 2.200Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTE:7-CHLOROTRYPTOPHAN'>CTE</scene> | + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTE:7-CHLOROTRYPTOPHAN'>CTE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= PrnA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens]) | |GENE= PrnA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2apg|2APG]], [[2aqj|2AQJ]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ar8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ar8 OCA], [http://www.ebi.ac.uk/pdbsum/2ar8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ar8 RCSB]</span> | ||
}} | }} | ||
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[[Category: SSPF, Scottish Structural Proteomics Facility.]] | [[Category: SSPF, Scottish Structural Proteomics Facility.]] | ||
[[Category: Unversucht, S.]] | [[Category: Unversucht, S.]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: CTE]] | ||
| - | [[Category: FAD]] | ||
| - | [[Category: flavin-dependent halogenase]] | ||
[[Category: helical bundle]] | [[Category: helical bundle]] | ||
[[Category: sandwiched sheet]] | [[Category: sandwiched sheet]] | ||
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[[Category: sspf]] | [[Category: sspf]] | ||
[[Category: structural genomic]] | [[Category: structural genomic]] | ||
| - | [[Category: tryptophan 7-halogenase]] | + | [[Category: tryptophan 7-halogenase,flavin-dependent halogenase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:56:12 2008'' |
Revision as of 22:56, 30 March 2008
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| , resolution 2.200Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | PrnA (Pseudomonas fluorescens) | ||||||
| Related: | 2APG, 2AQJ
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The structure of tryptophan 7-halogenase (PrnA)suggests a mechanism for regioselective chlorination
Overview
Chlorinated natural products include vancomycin and cryptophycin A. Their biosynthesis involves regioselective chlorination by flavin-dependent halogenases. We report the structural characterization of tryptophan 7-halogenase (PrnA), which regioselectively chlorinates tryptophan. Tryptophan and flavin adenine dinucleotide (FAD) are separated by a 10 angstrom-long tunnel and bound by distinct enzyme modules. The FAD module is conserved in halogenases and is related to flavin-dependent monooxygenases. On the basis of biochemical studies, crystal structures, and by analogy with monooxygenases, we predict that FADH2 reacts with O2 to make peroxyflavin, which is decomposed by Cl-. The resulting HOCl is guided through the tunnel to tryptophan, where it is activated to participate in electrophilic aromatic substitution.
About this Structure
2AR8 is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.
Reference
Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination., Dong C, Flecks S, Unversucht S, Haupt C, van Pee KH, Naismith JH, Science. 2005 Sep 30;309(5744):2216-9. PMID:16195462
Page seeded by OCA on Mon Mar 31 01:56:12 2008
Categories: Pseudomonas fluorescens | Single protein | Dong, C. | Flecks, S. | Haupt, C. | Naismith, J H. | Pee, K H.Van. | SSPF, Scottish Structural Proteomics Facility. | Unversucht, S. | Helical bundle | Sandwiched sheet | Scottish structural proteomics facility | Sspf | Structural genomic | Tryptophan 7-halogenase,flavin-dependent halogenase
